Recombinant Human GADD45GIP1 Protein, N-His

Description of Recombinant Human GADD45GIP1 Protein, N-His

Introduction

Recombinant Human GADD45GIP1 Protein, also known as Growth Arrest and DNA Damage-Inducible Protein 45 Gamma-Interacting Protein 1, is a protein that plays a crucial role in cell cycle regulation and DNA damage response. This protein is produced through recombinant DNA technology, which involves inserting the gene for GADD45GIP1 into a host organism, such as bacteria, to produce large quantities of the protein for research and therapeutic purposes.

Structure of Recombinant Human GADD45GIP1 Protein

The Recombinant Human GADD45GIP1 Protein is a 17 kDa protein that consists of 151 amino acids. It contains a conserved N-terminal domain that is responsible for binding to the GADD45 family of proteins, as well as a C-terminal domain that is involved in protein-protein interactions. The protein also contains a nuclear localization signal, which allows it to be transported into the nucleus where it carries out its functions.

Activity of Recombinant Human GADD45GIP1 Protein

Recombinant Human GADD45GIP1 Protein is a multifunctional protein that plays a role in various cellular processes. It is primarily known for its involvement in the cell cycle, where it acts as a negative regulator of cell proliferation. It does this by binding to and inhibiting the activity of cyclin-dependent kinases, which are enzymes that promote cell division.

In addition to its role in the cell cycle, Recombinant Human GADD45GIP1 Protein is also involved in the DNA damage response. It is upregulated in response to DNA damage and helps to repair damaged DNA by activating the checkpoint kinase 1 (Chk1) pathway. This pathway is responsible for halting the cell cycle and allowing time for DNA repair to occur.

Furthermore, Recombinant Human GADD45GIP1 Protein has been shown to play a role in apoptosis, or programmed cell death. It promotes apoptosis by interacting with and activating the tumor suppressor protein p53, which is known to induce cell death in response to DNA damage.

Applications of Recombinant Human GADD45GIP1 Protein

Recombinant Human GADD45GIP1 Protein has a wide range of applications in both research and therapeutic settings. In research, it is commonly used as a tool to study cell cycle regulation and DNA damage response. Its ability to inhibit cell proliferation and promote apoptosis makes it a valuable tool for studying cancer and other diseases that involve abnormal cell growth.

In a therapeutic context, Recombinant Human GADD45GIP1 Protein has potential applications in cancer treatment. Its role in inhibiting cell proliferation and promoting apoptosis makes it a promising target for developing anti-cancer drugs. Additionally, its involvement in the DNA damage response makes it a potential candidate for enhancing the effectiveness of chemotherapy and radiation therapy.

Conclusion

In summary, Recombinant Human GADD45GIP1 Protein is a 17 kDa protein that plays a crucial role in cell cycle regulation and DNA damage response. Its structure consists of a conserved N-terminal domain, a C-terminal domain, and a nuclear localization signal. The protein is involved in various cellular processes, including cell cycle regulation, DNA repair, and apoptosis. Its multifunctional nature makes it a valuable tool for research and a potential candidate for cancer treatment.