Recombinant Human GSDMD, N-His

Introduction to Recombinant Human GSDMD

Recombinant Human GSDMD (Gasdermin D) is a protein that plays a crucial role in the innate immune response. It is a member of the gasdermin family, which is a group of proteins that are involved in various cellular processes, including cell death and inflammation. GSDMD is primarily expressed in immune cells such as macrophages, dendritic cells, and T cells, making it an essential component of the immune system.

Structure of Recombinant Human GSDMD

Recombinant Human GSDMD is a 48 kDa protein that is composed of 488 amino acids. It has a unique domain structure, consisting of an N-terminal domain, a linker region, and a C-terminal domain. The N-terminal domain of GSDMD is responsible for its activation, while the C-terminal domain is involved in its effector function.

Upon activation, GSDMD undergoes a conformational change, leading to the formation of pores in the cell membrane. These pores allow the release of pro-inflammatory cytokines and other cellular contents, contributing to the inflammatory response. The linker region of GSDMD is responsible for connecting the N-terminal and C-terminal domains, and it also plays a role in regulating the protein’s activity.

Activity of Recombinant Human GSDMD

The main activity of Recombinant Human GSDMD is its role in the formation of pores in the cell membrane. This process, known as pyroptosis, is a form of programmed cell death that is triggered by inflammatory signals. GSDMD is activated by inflammatory caspases, which cleave the protein at the linker region, leading to the release of the N-terminal domain. This domain then oligomerizes and forms pores in the cell membrane, causing the cell to swell and burst, releasing its contents.

In addition to its role in pyroptosis, GSDMD also plays a role in the release of pro-inflammatory cytokines, such as interleukin-1β (IL-1β) and interleukin-18 (IL-18). These cytokines are essential for the activation of the immune response and the recruitment of immune cells to the site of infection or injury.

Application of Recombinant Human GSDMD

Recombinant Human GSDMD has various applications in both research and clinical settings. In research, it is used to study the mechanisms of pyroptosis and its role in the immune response. It is also used to investigate the role of GSDMD in various diseases, such as inflammatory disorders and cancer.

In clinical settings, GSDMD has potential applications in the treatment of inflammatory diseases. As GSDMD is involved in the release of pro-inflammatory cytokines, targeting this protein could potentially reduce the inflammatory response and alleviate symptoms in diseases such as rheumatoid arthritis and sepsis.

Conclusion

Recombinant Human GSDMD is a crucial protein in the innate immune response. Its unique structure and role in pyroptosis make it an important target for research and potential therapeutic applications. As our understanding of GSDMD continues to grow, it may hold promise for the development of novel treatments for various inflammatory diseases.