Recombinant Human ING4 Protein, N-His

Description of Recombinant Human ING4 Protein, N-His

Introduction

Recombinant proteins have become an essential tool in the field of biotechnology, allowing for the production of large quantities of specific proteins for various research and therapeutic purposes. One such protein is the Recombinant Human ING4 Protein, which has gained significant attention in recent years due to its unique structure and potential applications. In this article, we will delve into the structure, activity, and applications of this protein.

Structure of Recombinant Human ING4 Protein

The ING4 gene encodes for the ING4 protein, which is a member of the inhibitor of growth (ING) family. The human ING4 protein is composed of 346 amino acids and has a molecular weight of approximately 38 kDa. It consists of a highly conserved PHD (plant homeodomain) domain and a nuclear localization signal (NLS) at its N-terminus, followed by a central region and a C-terminal leucine zipper motif.

The PHD domain of ING4 is responsible for its binding to histone H3, which plays a crucial role in regulating gene expression. The NLS allows for the protein’s transportation into the nucleus, where it exerts its functions. The central region contains a proline-rich domain, which is involved in protein-protein interactions, and a serine-rich region, which is a potential phosphorylation site.

Activity of Recombinant Human ING4 Protein

The ING4 protein is a tumor suppressor that regulates various cellular processes, including cell proliferation, apoptosis, and DNA repair. It exerts its functions by interacting with various proteins, such as p53, p300, and HDAC1, and modulating their activities.

One of the key roles of ING4 is its ability to induce apoptosis, or programmed cell death, in cancer cells. It does so by activating the p53 pathway, which is a critical regulator of cell cycle arrest and apoptosis. ING4 also inhibits the activity of HDAC1, a histone deacetylase that is overexpressed in many cancers, leading to the suppression of tumor growth.

In addition to its tumor-suppressive activity, ING4 also plays a role in DNA repair. It interacts with DNA repair proteins, such as BRCA1 and RAD51, and promotes the repair of DNA damage, thus preventing the accumulation of mutations that can lead to cancer development.

Applications of Recombinant Human ING4 Protein

The unique structure and activity of ING4 make it a promising candidate for various applications in the field of biotechnology and medicine. Some potential applications of Recombinant Human ING4 Protein include:

Cancer Therapy

As a tumor suppressor, ING4 has shown promising results in inhibiting the growth of various cancer cells in vitro and in vivo. Its ability to induce apoptosis and inhibit tumor growth makes it a potential candidate for cancer therapy. Recombinant Human ING4 Protein can be used as a therapeutic agent to treat various types of cancer, either alone or in combination with other anti-cancer drugs.

Gene Therapy

The unique structure of ING4 also makes it a suitable candidate for gene therapy. Its ability to regulate gene expression and modulate various cellular processes can be utilized to correct genetic disorders or treat diseases caused by abnormal gene expression.

Diagnostic Tool

The overexpression or underexpression of ING4 has been observed in various cancers, making it a potential diagnostic marker. Recombinant Human ING4 Protein can be used as a diagnostic tool to detect the levels of ING4 in patient samples, aiding in the early detection and diagnosis of cancer.

Research Tool

Recombinant Human ING4 Protein can also be used as a research tool to study its functions and interactions with other proteins. Its availability in large quantities allows for in-depth studies and further understanding of its role