Recombinant Human KIF7 Protein, N-His

Description of Recombinant Human KIF7 Protein, N-His

Introduction

The Recombinant Human KIF7 Protein is a key protein involved in cellular transport and signaling pathways. It belongs to the kinesin family of proteins, which are molecular motors responsible for transporting cargo along microtubules within the cell. KIF7 has been extensively studied for its role in development and disease, and the availability of recombinant protein has greatly aided in these investigations.

Structure of Recombinant Human KIF7 Protein

The Recombinant Human KIF7 Protein is a 170 kDa protein with a conserved motor domain at its N-terminus. This domain contains the ATP binding and hydrolysis sites, which are essential for the motor function of kinesins. The motor domain is followed by a coiled-coil region, which is responsible for dimerization of the protein. At the C-terminus, KIF7 has a globular domain that is involved in binding to microtubules and cargo molecules.

Activity of Recombinant Human KIF7 Protein

The primary function of KIF7 is to transport cargo along microtubules, which are dynamic structures that provide the cellular framework for intracellular transport. KIF7 has been shown to play a critical role in the development of various tissues and organs, including the brain, limbs, and lungs. It is also involved in signaling pathways, such as the Hedgehog pathway, which is essential for embryonic development and tissue homeostasis.

KIF7 has been found to have both plus-end and minus-end directed motor activity, allowing it to transport cargo in both directions along the microtubule. It also has the ability to bind to different cargoes, such as vesicles, organelles, and other proteins, making it a versatile transporter within the cell.

Application of Recombinant Human KIF7 Protein

The availability of recombinant human KIF7 protein has greatly aided in the understanding of its structure and function. It has been used in various in vitro and in vivo studies to investigate its role in development and disease. Recombinant KIF7 has also been used in biochemical assays to study its motor activity and interactions with other proteins and molecules.

One of the major applications of recombinant KIF7 is in drug discovery and development. As KIF7 is involved in the Hedgehog signaling pathway, which is dysregulated in various cancers, it has been identified as a potential therapeutic target. Recombinant KIF7 protein can be used in high-throughput screening assays to identify small molecule inhibitors that can block its motor activity and disrupt the Hedgehog pathway.

Furthermore, recombinant human KIF7 protein has also been used in the production of antibodies for research and diagnostic purposes. As KIF7 is an antigen that is highly expressed in various tissues, recombinant protein can be used to generate specific antibodies that can be used to detect and study KIF7 in different biological samples.

Conclusion

The Recombinant Human KIF7 Protein is a crucial protein involved in cellular transport and signaling pathways. Its structure and function have been extensively studied, and the availability of recombinant protein has greatly aided in these investigations. With its diverse applications in research and drug development, recombinant KIF7 protein continues to play a vital role in advancing our understanding of its role in development and disease.