Recombinant Human LMOD3 Protein, N-His

Description of Recombinant Human LMOD3 Protein, N-His

Introduction to Recombinant Human LMOD3 Protein

Recombinant Human LMOD3 Protein is a highly purified, bioactive protein that is produced through recombinant DNA technology. This protein is encoded by the LMOD3 gene and is a member of the Leiomodin family. It plays a crucial role in the regulation of actin filament assembly, which is essential for various cellular processes such as cell movement, division, and shape maintenance.

Structure of Recombinant Human LMOD3 Protein

The Recombinant Human LMOD3 Protein is a 39 kDa protein consisting of 349 amino acids. It contains a central proline-rich region and two C-terminal tandem repeats of the WH2 domain, which is responsible for binding to actin monomers. The protein also has a highly conserved N-terminal region that is essential for its function in actin filament assembly.

Recombinant Human LMOD3 Protein is produced in a mammalian expression system, ensuring proper folding and post-translational modifications, such as glycosylation, that are crucial for its activity. The purity of the protein is greater than 95%, making it suitable for various applications in research and drug development.

Activity of Recombinant Human LMOD3 Protein

The primary function of Recombinant Human LMOD3 Protein is to regulate actin filament assembly. It binds to actin monomers and promotes their polymerization into long filaments, which are essential for cell movement and shape maintenance. This activity is mediated through its WH2 domains, which interact with actin monomers and facilitate their addition to the growing filament.

Studies have also shown that Recombinant Human LMOD3 Protein can regulate actin dynamics by inhibiting the binding of capping proteins to the barbed end of actin filaments. This allows for continuous filament elongation, which is crucial for processes such as cell motility and cytokinesis.

Moreover, Recombinant Human LMOD3 Protein has been found to interact with other proteins involved in actin regulation, such as tropomyosin and cofilin. These interactions further enhance the protein’s ability to regulate actin filament assembly and dynamics.

Application of Recombinant Human LMOD3 Protein

Recombinant Human LMOD3 Protein has a wide range of applications in both basic research and drug development. Its role in actin regulation makes it a valuable tool for studying cellular processes such as cell migration, division, and morphogenesis. The protein can be used to investigate the mechanisms underlying these processes and to identify potential therapeutic targets for diseases related to actin dysfunction.

Furthermore, Recombinant Human LMOD3 Protein has potential therapeutic applications in treating diseases such as cancer and cardiovascular disorders. As actin dynamics play a crucial role in tumor invasion and metastasis, targeting LMOD3 could potentially inhibit these processes and prevent cancer progression. In cardiovascular diseases, LMOD3 has been found to regulate the contractility of smooth muscle cells, making it a potential target for the treatment of conditions such as hypertension and atherosclerosis.

In addition, Recombinant Human LMOD3 Protein can be used in drug screening assays to identify compounds that modulate its activity. This can aid in the development of novel drugs that target actin dynamics and have therapeutic potential in various diseases.

Conclusion

In summary, Recombinant Human LMOD3 Protein is a bioactive protein that plays a crucial role in regulating actin filament assembly. Its structure, activity, and potential applications make it a valuable tool for studying cellular processes and developing novel therapeutics. With further research and development, this protein has the potential to make a significant impact in the fields of basic science and medicine.