Recombinant Human NOTCH4, N-His

Description of Recombinant Human NOTCH4, N-His

Introduction

Recombinant Human NOTCH4, also known as neurogenic locus notch homolog protein 4, is a transmembrane protein that plays a crucial role in cell signaling and development. This protein is a member of the Notch family, which consists of four receptors (NOTCH1-4) and five ligands (JAG1, JAG2, DLL1, DLL3, and DLL4). NOTCH4 is essential for the proper functioning of various tissues and organs, including the brain, heart, and immune system.

Structure of Recombinant Human NOTCH4

The NOTCH4 gene is located on chromosome 6 in humans and encodes for a 2001 amino acid long protein. The protein consists of an extracellular domain, a transmembrane domain, and an intracellular domain. The extracellular domain contains multiple epidermal growth factor (EGF)-like repeats, which are responsible for ligand binding. The transmembrane domain anchors the protein to the cell membrane, and the intracellular domain contains a transcriptional activation domain.

The recombinant form of NOTCH4 is produced by cloning the NOTCH4 gene into a suitable expression vector and then expressing it in a host organism, such as bacteria or mammalian cells. This allows for the production of large quantities of pure and functional protein for research and therapeutic purposes.

Activity of Recombinant Human NOTCH4

NOTCH4 is primarily involved in cell-to-cell communication, specifically in the Notch signaling pathway. This pathway plays a crucial role in the development and maintenance of various tissues and organs. NOTCH4 is activated by binding to its ligands, which leads to a series of proteolytic cleavages and the release of the intracellular domain. The intracellular domain then translocates to the nucleus, where it acts as a transcription factor and regulates the expression of target genes.

NOTCH4 has been shown to play a role in various cellular processes, including cell proliferation, differentiation, and apoptosis. It is also involved in the development of the nervous system, cardiovascular system, and immune system. Dysregulation of NOTCH4 activity has been linked to various diseases, such as cancer, cardiovascular diseases, and neurological disorders.

Application of Recombinant Human NOTCH4

The recombinant form of NOTCH4 has a wide range of applications in both research and therapeutics. In research, it is used to study the structure and function of NOTCH4 and its role in various cellular processes. It is also used to screen for potential ligands and inhibitors of NOTCH4, which can aid in the development of new drugs for diseases associated with NOTCH4 dysregulation.

In therapeutics, recombinant NOTCH4 is being investigated as a potential treatment for various diseases. For example, in cancer, NOTCH4 is overexpressed in certain types of tumors, and targeting NOTCH4 activity has shown promising results in inhibiting tumor growth. In cardiovascular diseases, NOTCH4 has been linked to the development of atherosclerosis, and targeting NOTCH4 activity may help prevent or treat this condition. Additionally, NOTCH4 has been shown to play a role in neurological disorders, such as Alzheimer’s disease, and targeting its activity may have therapeutic potential in these conditions.

Conclusion

In summary, Recombinant Human NOTCH4 is a crucial protein involved in cell signaling and development. Its structure, activity, and application have been extensively studied and have provided valuable insights into its role in various cellular processes and diseases. The availability of recombinant NOTCH4 has greatly aided in the understanding and potential treatment of diseases associated with NOTCH4 dysregulation.