Recombinant Human PIN1 Protein, N-GST

Description of Recombinant Human PIN1 Protein, N-GST

Introduction

Recombinant Human PIN1 Protein is a highly versatile and important protein in the field of molecular biology. It is a recombinant protein that is produced through genetic engineering techniques, making it a valuable tool for studying and understanding various biological processes. In this article, we will discuss the structure, activity, and applications of this protein.

Structure of Recombinant Human PIN1 Protein

The Recombinant Human PIN1 Protein is a 163 amino acid protein with a molecular weight of approximately 18 kDa. It is composed of two domains: an N-terminal WW domain and a C-terminal PPIase domain. The WW domain is responsible for binding to proline-rich motifs in target proteins, while the PPIase domain has peptidyl-prolyl cis-trans isomerase activity, which is crucial for its function.

Activity of Recombinant Human PIN1 Protein

The main function of Recombinant Human PIN1 Protein is to regulate the conformational changes of target proteins by catalyzing the cis-trans isomerization of proline residues. This activity is essential for many cellular processes such as cell cycle progression, DNA damage response, and protein folding. PIN1 has been shown to interact with a wide range of proteins, including transcription factors, kinases, and tumor suppressors, and can modulate their activity and stability.

Applications of Recombinant Human PIN1 Protein

Recombinant Human PIN1 Protein has a variety of applications in both research and medicine. Its ability to regulate protein conformational changes makes it a valuable tool for studying protein-protein interactions and signaling pathways. It has also been used in drug discovery and development, as PIN1 is involved in many diseases, including cancer and Alzheimer’s disease.

1. Role in Cancer

PIN1 has been shown to play a critical role in cancer development and progression. It is overexpressed in many types of cancer and has been linked to increased cell proliferation, survival, and invasion. Inhibition of PIN1 activity has been proposed as a potential therapeutic strategy for cancer treatment.

2. Role in Alzheimer’s Disease

PIN1 has also been implicated in the development of Alzheimer’s disease. It has been shown to regulate the activity of tau protein, which is a major component of the neurofibrillary tangles found in the brains of Alzheimer’s patients. Inhibition of PIN1 has been suggested as a potential treatment for this neurodegenerative disease.

3. Diagnostic Marker

PIN1 has been identified as a potential diagnostic marker for various diseases. Its overexpression has been associated with poor prognosis in cancer patients, and it has also been found to be elevated in patients with Alzheimer’s disease. Detection of PIN1 levels in patient samples could aid in early diagnosis and treatment of these diseases.

4. Protein Engineering

The recombinant nature of PIN1 allows for protein engineering techniques to be used to modify its structure and activity. This has led to the development of PIN1 mutants with altered substrate specificity and enhanced stability, which can be used in various research applications.

5. Cell Signaling Studies

PIN1 is involved in many signaling pathways, and its recombinant form has been used to study these pathways in detail. By understanding how PIN1 interacts with its target proteins and modulates their activity, we can gain insight into the complex mechanisms of cell signaling.

6. Protein-Protein Interaction Studies</h