Recombinant Human PITRM1, N-His

Description of Recombinant Human PITRM1, N-His

Title: Introduction to Recombinant Human PITRM1

Recombinant Human PITRM1, also known as Presequence Protease, is a protein that plays a crucial role in the maintenance of mitochondrial function. It is a member of the M16 metallopeptidase family and is involved in the degradation of presequences of precursor proteins targeted to the mitochondria. In this article, we will explore the structure, activity, and application of Recombinant Human PITRM1.

Title: Structure of Recombinant Human PITRM1

Recombinant Human PITRM1 is a 100 kDa protein that contains 912 amino acids. It is composed of three domains: an N-terminal mitochondrial targeting sequence, a central catalytic domain, and a C-terminal domain. The catalytic domain is responsible for the proteolytic activity of PITRM1, while the C-terminal domain is involved in protein-protein interactions.

The catalytic domain of PITRM1 contains a highly conserved metallopeptidase motif, HEXXH, which is responsible for the hydrolysis of peptide bonds. This domain also contains a zinc-binding site, which is essential for the catalytic activity of PITRM1. The C-terminal domain of PITRM1 contains a coiled-coil motif, which is involved in the formation of homo-oligomers.

Title: Activity of Recombinant Human PITRM1

Recombinant Human PITRM1 is a highly specific protease that cleaves presequences of precursor proteins targeted to the mitochondria. It recognizes and cleaves peptides with a presequence motif of -[R/K]-[F/Y]-[L/I/V]-[K/R]-[H/Q]-[L/I/V]-[S/A]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-[L/I/V]-[L/I/V]-[A/G]-