Recombinant Human PLOD3 Protein, N-His

Description of Recombinant Human PLOD3 Protein, N-His

Introduction

Recombinant Human PLOD3 Protein, also known as procollagen-lysine, 2-oxoglutarate 5-dioxygenase 3 (PLOD3), is a protein that plays a vital role in collagen biosynthesis. It is a member of the PLOD family of proteins, which are responsible for the hydroxylation of lysine residues in collagen molecules. PLOD3 is a key enzyme in the formation of collagen cross-links, which are essential for the stability and strength of collagen fibers. In this article, we will discuss the structure, activity, and applications of Recombinant Human PLOD3 Protein.

Structure of Recombinant Human PLOD3 Protein

Recombinant Human PLOD3 Protein is a 73 kDa protein that consists of 662 amino acids. It has a conserved domain known as the Fe(II) and 2-oxoglutarate-dependent dioxygenase (2OGDO) domain, which is responsible for its enzymatic activity. This domain contains two histidine residues that are essential for binding to iron and 2-oxoglutarate, which are required for the hydroxylation reaction.

Activity of Recombinant Human PLOD3 Protein

The main activity of Recombinant Human PLOD3 Protein is the hydroxylation of lysine residues in collagen molecules. This process involves the insertion of a hydroxyl group into the lysine residue, which results in the formation of hydroxylysine. Hydroxylysine is an important component of collagen cross-links, which are crucial for the stability and strength of collagen fibers.

Recombinant Human PLOD3 Protein has been shown to have a preference for lysine residues in the Xaa-Lys-Gly sequence, which is commonly found in the collagen triple helix. This sequence is essential for the formation of collagen fibers, and the hydroxylation of lysine in this sequence is critical for the proper functioning of collagen.

In addition to its hydroxylation activity, Recombinant Human PLOD3 Protein has also been shown to have prolyl-4-hydroxylase activity. This activity is responsible for the hydroxylation of proline residues in collagen, which is important for the stability and folding of collagen molecules.

Applications of Recombinant Human PLOD3 Protein

Recombinant Human PLOD3 Protein has a wide range of applications in both research and industry. Its main application is in the production of recombinant collagen for tissue engineering and regenerative medicine. The hydroxylation of lysine and proline residues by PLOD3 is crucial for the proper folding and stability of collagen, making it an essential component in the production of functional collagen for tissue repair and regeneration.

In addition, Recombinant Human PLOD3 Protein has also been used in the production of recombinant collagen-based biomaterials for wound healing, drug delivery, and cosmetic applications. Its ability to hydroxylate lysine and proline residues in collagen can be harnessed to create collagen-based materials with specific properties and functionalities.

Furthermore, Recombinant Human PLOD3 Protein has been studied for its potential role in cancer progression. It has been found to be overexpressed in various types of cancer, and its activity has been linked to tumor growth and metastasis. This makes PLOD3 a potential target for cancer therapy, and further research is being conducted to understand its role in cancer and develop targeted treatments.

Conclusion

Recombinant Human PLOD3 Protein is a crucial enzyme involved in collagen biosynthesis. Its hydroxylation activity plays a vital role in the stability and strength of collagen fibers, making it an essential component in tissue engineering and regenerative medicine. Its applications also extend to the production of collagen-based biomaterials and its potential role in cancer therapy. Further research on Recombinant Human PLOD3 Protein will continue to uncover its importance and