Recombinant Human PPP1R15B Protein, N-GST & C-His

Description of Recombinant Human PPP1R15B Protein, N-GST & C-His

Introduction to Recombinant Human PPP1R15B Protein

Recombinant Human PPP1R15B Protein, also known as protein phosphatase 1 regulatory subunit 15B, is a highly conserved protein that plays a crucial role in regulating cellular response to stress. This protein is encoded by the PPP1R15B gene and is a member of the PPP1R15 family of proteins.

Structure of Recombinant Human PPP1R15B Protein

The Recombinant Human PPP1R15B Protein is a 203 amino acid long protein with a molecular weight of approximately 23 kDa. It contains a highly conserved C-terminal domain that is responsible for its interaction with protein phosphatase 1 (PP1) and a variable N-terminal domain that is involved in its regulatory functions.

The crystal structure of Recombinant Human PPP1R15B Protein has been determined, revealing a compact globular structure with a central beta-sheet surrounded by alpha-helices. This structure is highly similar to other members of the PPP1R15 family, indicating a conserved function among these proteins.

Activity of Recombinant Human PPP1R15B Protein

Recombinant Human PPP1R15B Protein is a key regulator of the integrated stress response (ISR) pathway. This pathway is activated in response to various stressors such as nutrient deprivation, hypoxia, and viral infection. The primary function of PPP1R15B is to inhibit protein synthesis by dephosphorylating eukaryotic initiation factor 2 alpha (eIF2α), a key regulator of translation initiation.

Under normal conditions, eIF2α is phosphorylated by kinases in response to stress, leading to a decrease in protein synthesis and conservation of cellular resources. However, prolonged eIF2α phosphorylation can have detrimental effects on cell survival. This is where Recombinant Human PPP1R15B Protein comes into play. It acts as a negative regulator of eIF2α phosphorylation, preventing excessive inhibition of protein synthesis and promoting cell survival.

Application of Recombinant Human PPP1R15B Protein

Recombinant Human PPP1R15B Protein has been extensively studied for its role in the ISR pathway and its potential applications in various fields. One of the major applications of this protein is in cancer research. The ISR pathway is often dysregulated in cancer cells, leading to increased protein synthesis and cell proliferation. By targeting PPP1R15B, researchers hope to develop new therapies for cancer treatment.

Another potential application of Recombinant Human PPP1R15B Protein is in the treatment of neurodegenerative diseases. Studies have shown that the ISR pathway is also involved in the pathogenesis of diseases such as Alzheimer’s and Parkinson’s. By targeting PPP1R15B, researchers aim to modulate the ISR pathway and potentially slow down the progression of these diseases.

Recombinant Human PPP1R15B Protein also has potential applications in the field of virology. Viruses often manipulate the ISR pathway for their own benefit, allowing them to replicate and spread. By targeting PPP1R15B, researchers hope to develop new antiviral therapies that can inhibit viral replication and prevent the spread of infection.

Conclusion

In summary, Recombinant Human PPP1R15B Protein is a highly conserved protein that plays a crucial role in regulating the ISR pathway. Its structure, activity, and potential applications make it a valuable protein for research in various fields, including cancer, neurodegenerative diseases, and virology. Further studies on this protein may lead to the development of new therapies for these diseases and provide a better understanding of the complex mechanisms involved in cellular stress response.