Recombinant Human PRKCG Protein, N-His

Description of Recombinant Human PRKCG Protein, N-His

Title: Introduction to Recombinant Human PRKCG Protein

Recombinant human PRKCG protein, also known as protein kinase C gamma (PRKCG), is a key enzyme involved in various cellular signaling pathways. It is a member of the protein kinase C (PKC) family and plays a crucial role in regulating cell growth, survival, and differentiation. This protein is highly conserved across species and is expressed in various tissues, including the brain, heart, and immune cells. In this article, we will explore the structure, activity, and applications of recombinant human PRKCG protein.

Title: Structure of Recombinant Human PRKCG Protein

The PRKCG gene encodes for a protein of 697 amino acids, with a molecular weight of approximately 78 kDa. The recombinant human PRKCG protein is produced in a bacterial expression system and purified to homogeneity. It consists of a regulatory domain, a catalytic domain, and a C-terminal tail. The regulatory domain contains two C1 domains, which bind to the lipid second messenger diacylglycerol (DAG) and the calcium ion, respectively. The catalytic domain contains a conserved ATP-binding site and a substrate-binding site. The C-terminal tail contains a hydrophobic region, which anchors the protein to the cell membrane.

Title: Activity of Recombinant Human PRKCG Protein

Recombinant human PRKCG protein is a serine/threonine kinase, which means it phosphorylates target proteins on serine or threonine residues. It is activated by binding to DAG and calcium ions, which leads to a conformational change and exposure of the catalytic domain. Once activated, PRKCG phosphorylates a wide range of substrates, including other kinases, transcription factors, and cytoskeletal proteins. This phosphorylation cascade ultimately leads to the activation of downstream signaling pathways, which regulate various cellular processes.

Title: Applications of Recombinant Human PRKCG Protein

Recombinant human PRKCG protein has been widely used in research to study its role in cellular signaling and its potential as a therapeutic target. It has been shown to play a critical role in cell proliferation, differentiation, and survival, making it an attractive target for cancer therapy. In fact, several small molecule inhibitors of PRKCG have been developed and are currently being evaluated in clinical trials for the treatment of various cancers.

Moreover, recombinant human PRKCG protein has also been used to study its role in neurological disorders such as Alzheimer’s disease and Parkinson’s disease. It has been shown to be involved in the regulation of synaptic plasticity and memory formation, making it a potential target for the treatment of these diseases.

Additionally, recombinant human PRKCG protein has been used in the development of diagnostic assays for autoimmune diseases. It has been identified as a target antigen in autoimmune disorders such as systemic lupus erythematosus and multiple sclerosis. Antibodies against PRKCG have been detected in patients with these diseases, making it a potential biomarker for diagnosis and disease monitoring.

Title: Conclusion

Recombinant human PRKCG protein is a crucial enzyme involved in various cellular processes and has been extensively studied for its potential as a therapeutic target. Its structure, activity, and applications have been well characterized, making it a valuable tool in research and drug development. With ongoing studies and clinical trials, the role of PRKCG in disease pathogenesis and its potential as a therapeutic target will continue to be explored, paving the way for new treatments and diagnostic tools.