Recombinant Human PSMD11 Protein, N-His

Description of Recombinant Human PSMD11 Protein, N-His

Title: Introduction to Recombinant Human PSMD11 Protein

Recombinant Human PSMD11 Protein, also known as proteasome 26S subunit, non-ATPase 11 (PSMD11), is a protein that plays a crucial role in the regulation of protein degradation within cells. This protein is a part of the 26S proteasome complex, which is responsible for the degradation of damaged or unwanted proteins in the cell. Recombinant human PSMD11 protein is widely used in various research and biotechnology applications due to its structural and functional properties.

Title: Structure of Recombinant Human PSMD11 Protein

Recombinant Human PSMD11 Protein is a 40 kDa protein that is composed of 364 amino acids. It is a non-ATPase subunit of the 26S proteasome complex and is highly conserved across different species. The protein consists of a N-terminal domain and a C-terminal domain, which are connected by a flexible linker region. The N-terminal domain contains a ubiquitin-like (UBL) domain, which is responsible for the interaction with other proteins. The C-terminal domain contains a proteasome-COP9 signalosome (CSN) regulatory subunit (PCS) domain, which is involved in the regulation of the proteasome complex.

Title: Activity of Recombinant Human PSMD11 Protein

Recombinant Human PSMD11 Protein has been shown to play a crucial role in the assembly and function of the 26S proteasome complex. It acts as a scaffolding protein, bringing together the different subunits of the complex and facilitating their proper assembly. The protein also has a regulatory function, as it interacts with other proteins to control the activity of the proteasome complex. Additionally, PSMD11 has been found to be involved in the recognition and degradation of specific proteins, such as misfolded or damaged proteins, through its interaction with the UBL domain.

Title: Applications of Recombinant Human PSMD11 Protein

Recombinant Human PSMD11 Protein has a wide range of applications in the field of research and biotechnology. Its role in protein degradation makes it a valuable tool for studying cellular processes, such as protein turnover and quality control. It is also used in the production of recombinant proteins, as it can enhance the efficiency of protein expression and folding. Furthermore, PSMD11 has been implicated in various diseases, such as cancer and neurodegenerative disorders, making it a potential therapeutic target.

Title: Recombinant Human PSMD11 Protein as an Antigen

Recombinant Human PSMD11 Protein can also be used as an antigen in immunological assays. The UBL domain of PSMD11 has been shown to be immunogenic, eliciting a strong antibody response in animals. This makes it a useful antigen for the production of specific antibodies for research and diagnostic purposes. Additionally, PSMD11 has been identified as a potential tumor antigen, as it is overexpressed in certain types of cancer. This makes it a promising target for cancer immunotherapy.

Title: Production of Recombinant Human PSMD11 Protein

Recombinant Human PSMD11 Protein can be produced using recombinant DNA technology. The gene encoding PSMD11 is cloned into an expression vector and introduced into a suitable host cell, such as E. coli or mammalian cells. The protein is then expressed and purified using various techniques, such as affinity chromatography. Recombinant PSMD11 protein can be produced in large quantities, making it readily available for research and commercial use.

In conclusion, Recombinant Human PSMD11 Protein is a highly versatile protein with important structural and functional properties. Its role in protein degradation and regulation makes it a valuable tool in various research and biotechnology applications. With its potential as an antigen and therapeutic target, PSMD11 continues to be a subject of interest in the scientific community.