Recombinant Human QKI Protein, N-His

Description of Recombinant Human QKI Protein, N-His

Introduction

Recombinant Human QKI Protein, also known as Quaking protein, is a member of the STAR (signal transduction and activation of RNA) family of RNA-binding proteins. It plays a crucial role in the regulation of RNA processing, stability, and translation, making it a vital protein in various biological processes.

Structure of Recombinant Human QKI Protein

Recombinant Human QKI Protein is a 378 amino acid protein with a molecular weight of approximately 42 kDa. It is composed of three main domains: an N-terminal STAR domain, a central KH domain, and a C-terminal QUA1 domain. The STAR domain is responsible for binding to RNA, while the KH domain is involved in protein-protein interactions. The QUA1 domain is responsible for the protein’s oligomerization, which is crucial for its function.

Activity of Recombinant Human QKI Protein

Recombinant Human QKI Protein is an RNA-binding protein that plays a crucial role in regulating RNA processing, stability, and translation. It binds to specific RNA sequences and controls their splicing, transport, and translation. QKI protein has been found to regulate the expression of numerous genes involved in cell proliferation, differentiation, and apoptosis. It also plays a role in myelination, neuronal development, and synaptic plasticity.

One of the key activities of Recombinant Human QKI Protein is its role in alternative splicing. It binds to pre-mRNA sequences and regulates the splicing of exons, leading to the production of different isoforms of a protein. This process is critical for the diversity of proteins that can be produced from a single gene.

Recombinant Human QKI Protein also plays a crucial role in RNA stability. It binds to the 3′ untranslated region (UTR) of target mRNAs and regulates their stability. This activity is essential for the proper expression of genes involved in cell growth, differentiation, and survival.

Another important activity of Recombinant Human QKI Protein is its role in translational regulation. It binds to specific sequences in the 5′ UTR of target mRNAs and controls their translation. This activity is crucial for the proper expression of genes involved in neuronal development and synaptic plasticity.

Application of Recombinant Human QKI Protein

Recombinant Human QKI Protein has a wide range of applications in both basic and clinical research. Its role in regulating RNA processing, stability, and translation makes it a valuable tool for studying gene expression and its dysregulation in various diseases.

One of the key applications of Recombinant Human QKI Protein is in the study of alternative splicing. Its ability to regulate the splicing of exons makes it a valuable tool for investigating the role of alternative splicing in various diseases, such as cancer and neurodegenerative disorders.

Recombinant Human QKI Protein is also used in the study of RNA stability. Its role in regulating the stability of target mRNAs makes it a valuable tool for investigating the mechanisms underlying RNA degradation and its dysregulation in diseases such as cancer.

In addition, Recombinant Human QKI Protein is used in the study of translational regulation. Its ability to regulate the translation of specific mRNAs makes it a valuable tool for investigating the role of translation in various cellular processes and diseases.

Furthermore, Recombinant Human QKI Protein has potential therapeutic applications. Its role in regulating gene expression and its dysregulation in diseases make it a promising target for developing therapies. For example, QKI protein has been found to be downregulated in various cancers, and its restoration has been shown to inhibit tumor growth, making it a potential target for cancer therapy.

Conclusion

Recombinant Human QKI Protein is a crucial protein involved in the regulation of RNA processing, stability, and translation. Its structure, activity, and applications make it a valuable tool for studying gene expression and its dysregulation in various diseases. As research on QKI protein continues, its potential in both basic and clinical research is