Recombinant Human RAB27B Protein, N-His

Description of Recombinant Human RAB27B Protein, N-His

Introduction to Recombinant Human RAB27B Protein

Recombinant Human RAB27B Protein, also known as Ras-related protein Rab-27B, is a member of the small GTPase superfamily. This protein is encoded by the RAB27B gene and is involved in the regulation of intracellular vesicle trafficking and secretion. It is a key player in various cellular processes such as exocytosis, endocytosis, and organelle transport. Recombinant Human RAB27B Protein has been extensively studied for its structure, activity, and applications in various fields of research.

Structure of Recombinant Human RAB27B Protein

The recombinant form of RAB27B protein is produced by expressing the RAB27B gene in a suitable host organism such as bacteria or yeast. The resulting protein is a 25 kDa protein consisting of 219 amino acids. It contains a conserved GTPase domain, which enables it to bind to and hydrolyze GTP, and a C-terminal hypervariable region that is responsible for its specific targeting to different cellular compartments.

Structural studies have revealed that Recombinant Human RAB27B Protein exists in two distinct conformations – the GDP-bound inactive form and the GTP-bound active form. In its active form, the protein undergoes a conformational change, exposing its effector binding site, which allows it to interact with downstream effectors and regulate various cellular processes.

Activity of Recombinant Human RAB27B Protein

Recombinant Human RAB27B Protein plays a crucial role in regulating the transport and secretion of vesicles in cells. It is primarily involved in the exocytosis of secretory granules, which are responsible for the release of hormones, neurotransmitters, and other signaling molecules. This protein also regulates the trafficking of lysosomes, which are responsible for degrading and recycling cellular waste products.

Studies have shown that Recombinant Human RAB27B Protein interacts with a variety of effector proteins, including myosin motors, tethering factors, and SNARE proteins, to control vesicle transport and fusion. It also plays a role in the formation and maintenance of specialized vesicular structures, such as melanosomes in melanocytes and lytic granules in cytotoxic T cells.

Applications of Recombinant Human RAB27B Protein

Recombinant Human RAB27B Protein has been widely used in various fields of research due to its diverse functions and potential applications. Some of the key applications of this protein are:

• Cell Biology: Recombinant Human RAB27B Protein has been extensively studied in cell biology to understand its role in vesicle trafficking and secretion. It has also been used to investigate the molecular mechanisms underlying various cellular processes, such as exocytosis, endocytosis, and organelle transport.
• Neuroscience: The role of Recombinant Human RAB27B Protein in regulating neurotransmitter release has made it a valuable tool in neuroscience research. It has been used to study the mechanisms of synaptic transmission and to explore potential therapeutic targets for neurological disorders.
• Cancer Research: Aberrant expression of RAB27B has been linked to various types of cancers, including breast, lung, and prostate cancer. Recombinant Human RAB27B Protein has been used to study the role of this protein in cancer progression and to identify potential targets for cancer therapy.
• Drug Development: The diverse functions of Recombinant Human RAB27B Protein make it a potential target for drug development. It has been used in high-throughput screening assays to identify small molecule inhibitors that can modulate its activity and potentially treat diseases associated with dysregulated vesicle trafficking.

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