Recombinant Human SLC30A7 Protein, N-GST & C-His

Description of Recombinant Human SLC30A7 Protein, N-GST & C-His

Introduction

Recombinant human SLC30A7 protein, also known as zinc transporter 7, is a membrane protein that plays a crucial role in maintaining zinc homeostasis in the body. This protein is encoded by the SLC30A7 gene and is expressed in various tissues, including the pancreas, liver, and brain. In this article, we will discuss the structure, activity, and applications of recombinant human SLC30A7 protein.

Structure of Recombinant Human SLC30A7 Protein

The recombinant human SLC30A7 protein is a transmembrane protein with a molecular weight of approximately 50 kDa. It is composed of 390 amino acids and has 8 transmembrane domains. The N- and C-termini of the protein are located in the cytoplasm, while the central region containing the transmembrane domains is embedded in the cell membrane.

The protein also contains a highly conserved histidine-rich region in the second transmembrane domain, which is responsible for binding and transporting zinc ions. This region is essential for the proper functioning of the protein.

Activity of Recombinant Human SLC30A7 Protein

Recombinant human SLC30A7 protein is a zinc transporter that plays a crucial role in maintaining zinc homeostasis in the body. It is primarily expressed in the cells of the pancreas, liver, and brain, where it regulates the uptake and distribution of zinc ions.

The protein functions as an antiporter, which means it transports zinc ions out of the cell in exchange for other ions such as protons. This process helps to regulate the intracellular concentration of zinc ions, which is essential for many cellular processes, including gene expression, enzyme activity, and cell signaling.

Moreover, studies have shown that recombinant human SLC30A7 protein is also involved in the regulation of insulin secretion in pancreatic beta cells. It is responsible for maintaining the proper levels of zinc ions in these cells, which is necessary for the production and release of insulin.

Applications of Recombinant Human SLC30A7 Protein

Recombinant human SLC30A7 protein has various applications in both research and therapeutic settings. One of the major applications of this protein is in studying zinc homeostasis and its role in various cellular processes. Researchers use recombinant SLC30A7 protein to study the function of this protein and its impact on zinc transport and distribution in different cell types.

Moreover, recombinant human SLC30A7 protein has potential therapeutic applications in treating diseases related to zinc deficiency. For instance, studies have shown that mutations in the SLC30A7 gene can lead to a rare genetic disorder called acrodermatitis enteropathica, which is characterized by severe zinc deficiency. In such cases, recombinant SLC30A7 protein can be used to supplement the deficient protein and restore normal zinc levels in the body.

Furthermore, the protein has also been studied as a potential target for the treatment of type 2 diabetes. As mentioned earlier, SLC30A7 plays a crucial role in regulating insulin secretion, and its dysfunction has been linked to impaired glucose metabolism. Therefore, recombinant human SLC30A7 protein could be used as a therapeutic agent to improve insulin secretion and glycemic control in diabetic patients.

Conclusion

In summary, recombinant human SLC30A7 protein is a zinc transporter that plays a critical role in maintaining zinc homeostasis in the body. It is composed of 390 amino acids and has 8 transmembrane domains. The protein functions as an antiporter and is involved in regulating zinc uptake and distribution in various tissues. Its applications range from studying zinc homeostasis to potential therapeutic interventions for diseases related to zinc deficiency and impaired insulin secretion. Further research on this protein could lead to a better understanding of its role in human health and the development of novel treatments for related disorders.