Recombinant Human SNX9, N-His

Description of Recombinant Human SNX9, N-His

Introduction

Recombinant Human SNX9 is a protein that plays a crucial role in regulating various cellular processes such as endocytosis, cell migration, and actin polymerization. It belongs to the sorting nexin (SNX) protein family, which is involved in the trafficking of proteins between cellular compartments. The recombinant form of SNX9 is produced in a laboratory setting using genetic engineering techniques, making it a valuable tool for studying its structure, activity, and applications.

Structure of Recombinant Human SNX9

The human SNX9 gene encodes for a protein of 590 amino acids, which is composed of several domains. The N-terminal region contains a phox homology (PX) domain, which is responsible for binding to phosphoinositides and targeting SNX9 to the plasma membrane. This is followed by a Bin/amphiphysin/Rvs (BAR) domain, which forms a crescent-shaped dimer that can sense and induce membrane curvature. The C-terminal region contains a Src homology 3 (SH3) domain, which mediates protein-protein interactions with various signaling molecules.

Recombinant Human SNX9 is produced by cloning the human SNX9 gene into a suitable expression vector and introducing it into host cells, such as bacteria or mammalian cells. The resulting protein is then purified using various chromatography techniques to obtain a highly pure and active form of SNX9.

Activity of Recombinant Human SNX9

The main function of SNX9 is to regulate the formation and trafficking of endocytic vesicles, which are responsible for internalizing various molecules from the cell surface. SNX9 interacts with other proteins, such as dynamin and actin, to promote the scission of endocytic vesicles from the plasma membrane. It also plays a role in actin polymerization, which is essential for cell migration and other cellular processes.

Recombinant Human SNX9 has been extensively studied for its role in endocytosis and actin dynamics. It has been shown to be involved in the formation of clathrin-coated pits, which are responsible for the internalization of various receptors and other molecules. SNX9 also regulates the maturation of early endosomes, which are responsible for sorting and trafficking of internalized molecules to their designated cellular compartments.

Applications of Recombinant Human SNX9

Recombinant Human SNX9 has numerous applications in both research and therapeutic settings. Its role in endocytosis and actin dynamics makes it a valuable tool for studying these cellular processes. Researchers can use recombinant SNX9 to investigate its interactions with other proteins and its effects on endocytosis and actin polymerization. It can also be used to study the role of SNX9 in various diseases, such as cancer and neurodegenerative disorders.

In therapeutic applications, SNX9 has been shown to play a role in the entry of certain viruses into host cells. Recombinant Human SNX9 can be used to block this entry, making it a potential antiviral therapy. Additionally, SNX9 has been implicated in the development of drug resistance in cancer cells. Targeting SNX9 using recombinant proteins or small molecule inhibitors could potentially overcome this resistance and improve the efficacy of chemotherapy drugs.

Conclusion

Recombinant Human SNX9 is a valuable protein with diverse functions in regulating cellular processes. Its structure, activity, and applications have been extensively studied, making it an important tool for researchers in various fields. Its role in endocytosis, actin dynamics, and disease makes it a promising target for therapeutic interventions. With ongoing research and advancements in genetic engineering techniques, the use of recombinant SNX9 is expected to further expand, providing a deeper understanding of its functions and potential for therapeutic applications.