Recombinant Human SRC Protein, N-His

Description of Recombinant Human SRC Protein, N-His

Introduction

Recombinant Human SRC Protein, also known as proto-oncogene tyrosine-protein kinase SRC, is a protein that plays a crucial role in cell signaling and regulation of various cellular processes. This protein is a member of the SRC family of non-receptor tyrosine kinases and is encoded by the SRC gene. In this article, we will discuss the structure, activity, and applications of recombinant human SRC protein.

Structure of Recombinant Human SRC Protein

Recombinant Human SRC Protein is composed of 536 amino acids and has a molecular weight of approximately 60 kDa. It consists of four distinct domains, including the Src homology 4 (SH4) domain, the SH3 domain, the SH2 domain, and the catalytic domain. The SH4 domain is responsible for membrane targeting, while the SH3 and SH2 domains are involved in protein-protein interactions. The catalytic domain contains the tyrosine kinase activity of the protein.

Activity of Recombinant Human SRC Protein

Recombinant Human SRC Protein is a non-receptor tyrosine kinase that plays a crucial role in the regulation of cell growth, differentiation, and survival. It is activated by various extracellular signals, such as growth factors, cytokines, and hormones. Upon activation, SRC protein phosphorylates its target proteins, leading to the activation of downstream signaling pathways. This protein is also involved in the regulation of cell adhesion, migration, and invasion, making it a key player in cancer progression.

Applications of Recombinant Human SRC Protein

Recombinant Human SRC Protein has various applications in both research and therapeutic settings. Some of the major applications of this protein are discussed below.

Cancer Research

SRC protein has been extensively studied in the context of cancer research. It has been found to be overexpressed and hyperactivated in many types of cancer, including breast, colon, and lung cancer. Recombinant Human SRC Protein is used in various in vitro and in vivo studies to understand its role in cancer progression and to develop novel therapeutic strategies targeting this protein.

Drug Development

Due to its crucial role in cancer and other diseases, SRC protein has emerged as a potential drug target. Recombinant Human SRC Protein is used in high-throughput screening assays to identify small molecule inhibitors that can block its activity. These inhibitors have the potential to be developed into anti-cancer drugs.

Protein-Protein Interaction Studies

The SH2 and SH3 domains of SRC protein are involved in protein-protein interactions, making it an important tool for studying these interactions. Recombinant Human SRC Protein is used in pull-down assays and co-immunoprecipitation experiments to identify and characterize its interacting partners.

Diagnostic Tool

Abnormal activation of SRC protein has been linked to various diseases, including cancer and osteoporosis. Recombinant Human SRC Protein is used in diagnostic tests to measure its activity in patient samples. This can aid in the early detection and monitoring of diseases associated with SRC protein.

Vaccine Development

Recombinant Human SRC Protein has also been studied as a potential antigen for vaccine development. This protein is overexpressed in cancer cells and is recognized by the immune system, making it a promising target for cancer vaccines. Further research is needed to explore its potential in this area.

Conclusion

In conclusion, Recombinant Human SRC Protein is a crucial player in cell signaling and regulation of cellular processes. Its structure, activity, and applications have been extensively studied, and it has emerged as a potential drug target and diagnostic tool. Further research on this protein may lead to the development of novel therapeutics for various diseases.