Recombinant Human TOR1B, N-His

Description of Recombinant Human TOR1B, N-His

Introduction

Recombinant Human TOR1B, N-His is a protein that plays a crucial role in various cellular processes such as protein folding, cell cycle regulation, and stress response. It is a promising drug target due to its involvement in several diseases, including cancer and neurodegenerative disorders. In this article, we will discuss the structure, activity, and potential applications of Recombinant Human TOR1B, N-His.

Structure of Recombinant Human TOR1B, N-His

Recombinant Human TOR1B, N-His is a 34 kDa protein consisting of 296 amino acids. It belongs to the AAA+ superfamily of ATPases and has a conserved ATP-binding domain. The protein has a unique N-terminal His tag, which allows for easy purification and detection. The crystal structure of Recombinant Human TOR1B, N-His has been determined, and it forms a homohexameric ring structure with a central pore.

Activity of this protein

Recombinant Human TOR1B, N-His is an ATP-dependent molecular chaperone that assists in the proper folding of newly synthesized proteins. It is also involved in the disaggregation of misfolded proteins and the prevention of protein aggregation. The protein has been shown to interact with various client proteins, including heat-shock proteins, transcription factors, and cytoskeletal proteins. Recombinant Human TOR1B, N-His uses the energy from ATP hydrolysis to exert mechanical force and unfold misfolded proteins, allowing them to refold correctly.

Role in Disease

Recombinant Human TOR1B, N-His has been implicated in several diseases, making it a promising drug target. In cancer, the overexpression of TOR1B has been observed in various types of tumors, and it has been shown to promote tumor growth and metastasis. In neurodegenerative disorders such as Parkinson’s and Huntington’s disease, TOR1B has been linked to protein misfolding and aggregation, which are hallmarks of these diseases. Additionally, studies have shown that TOR1B plays a role in viral infections, making it a potential target for antiviral therapies.

Potential Applications

Recombinant Human TOR1B, N-His has potential applications in both basic research and drug development. In basic research, it can be used as a tool to study protein folding and chaperone activity. Its unique N-terminal His tag allows for easy purification and detection, making it a valuable protein for biochemical and biophysical studies. In drug development, targeting TOR1B could lead to the development of novel therapies for cancer and neurodegenerative disorders. Inhibitors of TOR1B could potentially block its chaperone activity and prevent the growth and spread of tumors or reduce protein aggregation in neurodegenerative diseases.

Conclusion

In summary, Recombinant Human TOR1B, N-His is a 34 kDa protein that plays a crucial role in protein folding and has been implicated in various diseases. Its unique structure and activity make it a promising drug target for the development of novel therapies. Further research on this protein could lead to a better understanding of its role in disease and the development of targeted treatments.