Recombinant Human TP53/p53 Protein, N-GST

Description of Recombinant Human TP53/p53 Protein, N-GST

Recombinant Human TP53/p53 Protein: Structure, Activity, and Applications

The TP53 gene, also known as the p53 gene, is a tumor suppressor gene that plays a crucial role in regulating cell growth and preventing cancer development. Mutations in this gene have been linked to a variety of cancers, making it a key target for cancer research. Recombinant Human TP53/p53 protein, produced through genetic engineering techniques, has been widely used in scientific studies and clinical applications. In this article, we will explore the structure, activity, and applications of this important protein.

Structure of Recombinant Human TP53/p53 Protein

The TP53 gene encodes for a protein that consists of 393 amino acids. The recombinant human TP53/p53 protein is a full-length protein that is identical to the native protein found in humans. It is typically produced in bacterial or mammalian cell systems, using recombinant DNA technology. The protein is then purified and can be used for various applications.

The structure of the TP53 protein is composed of several domains, including a transactivation domain, a DNA-binding domain, and an oligomerization domain. These domains play important roles in the protein’s function, which we will discuss in the next section.

Activity of Recombinant Human TP53/p53 Protein

The main function of the TP53 protein is to act as a transcription factor, regulating the expression of genes involved in cell cycle control, DNA repair, and apoptosis. When DNA damage occurs, the TP53 protein is activated and binds to specific DNA sequences, promoting the expression of genes that halt cell division and initiate DNA repair. If the damage is too severe, the protein can also induce apoptosis, or programmed cell death, to prevent the proliferation of damaged cells.

Recombinant human TP53/p53 protein has been used extensively in research to study its activity and mechanisms of action. It has been shown to have a crucial role in tumor suppression, as mutations in the TP53 gene can lead to the loss of its tumor suppressive function and contribute to cancer development. In addition, the protein has been found to have anti-inflammatory and anti-aging effects, further highlighting its importance in maintaining cellular homeostasis.

Applications of Recombinant Human TP53/p53 Protein

The use of recombinant human TP53/p53 protein has expanded beyond research and into clinical applications. One of the most well-known applications is in cancer therapy. As mentioned earlier, mutations in the TP53 gene are commonly found in various types of cancer, making it an attractive target for treatment. Recombinant TP53 protein can be used as a therapeutic agent to restore the function of the mutated protein and induce tumor cell death.

In addition, recombinant TP53 protein has also been used in diagnostic tests for cancer. The detection of TP53 mutations in tumor cells can provide valuable information for cancer diagnosis and prognosis, as well as guide treatment decisions.

Furthermore, the use of recombinant human TP53/p53 protein in gene therapy has shown promising results. By delivering functional TP53 genes to cells with mutated or deleted TP53, gene therapy aims to restore the protein’s tumor suppressive function and potentially cure cancer.

Conclusion

In summary, recombinant human TP53/p53 protein is a crucial tool in the study of cancer and other diseases. Its structure, activity, and various applications have been extensively studied and continue to be explored. With its potential in cancer therapy and gene therapy, this protein holds great promise for the future of medical treatments. Further research and advancements in recombinant protein technology will undoubtedly continue to shed light on the role of TP53 in disease and pave the way for new treatments and cures.