Recombinant Human TRPM7/LTRPC7 Protein, N-His

Description of Recombinant Human TRPM7/LTRPC7 Protein, N-His

Introduction

The Recombinant Human TRPM7/LTRPC7 Protein is a highly sought-after protein in the field of molecular biology and biochemistry. It is a synthetic version of the human TRPM7/LTRPC7 protein, which is a member of the transient receptor potential (TRP) family of ion channels. This protein is involved in a variety of cellular functions, including cell proliferation, migration, and survival. In this article, we will discuss the structure, activity, and applications of this important recombinant protein.

Structure of Recombinant Human TRPM7/LTRPC7 Protein

The recombinant human TRPM7/LTRPC7 protein is a large protein with a molecular weight of approximately 200 kDa. It is composed of two main domains – an N-terminal cytoplasmic domain and a C-terminal transmembrane domain. The N-terminal domain contains several functional motifs, including a kinase domain and a coiled-coil domain, while the transmembrane domain is responsible for the ion channel activity of the protein.

The recombinant protein is produced using recombinant DNA technology, where the gene for TRPM7/LTRPC7 is inserted into a host organism, such as bacteria or mammalian cells. This allows for the large-scale production of the protein in a controlled and efficient manner.

Activity of Recombinant Human TRPM7/LTRPC7 Protein

The main activity of the recombinant human TRPM7/LTRPC7 protein is its role as an ion channel. This protein forms a non-selective cation channel that allows the passage of various ions, including calcium, magnesium, and zinc. The activity of this ion channel is crucial for maintaining cellular homeostasis and regulating various cellular processes.

In addition to its ion channel activity, the recombinant protein also has kinase activity, which is important for regulating cell signaling pathways. This activity is mediated by the kinase domain in the N-terminal region of the protein.

Applications of Recombinant Human TRPM7/LTRPC7 Protein

The recombinant human TRPM7/LTRPC7 protein has a wide range of applications in both research and therapeutic settings. Some of the key applications include:

• Functional studies: The recombinant protein is commonly used in functional studies to understand the role of TRPM7/LTRPC7 in various cellular processes. By manipulating the expression or activity of the protein, researchers can gain insights into its function and potential therapeutic targets.
• Drug discovery: The ion channel activity of TRPM7/LTRPC7 makes it an attractive target for drug discovery. Researchers can use the recombinant protein to screen for potential inhibitors or activators of the protein, which can then be further developed into therapeutic drugs.
• Diagnostic tool: The recombinant protein can also be used as a diagnostic tool in certain diseases. For example, mutations in the TRPM7 gene have been linked to certain neurological disorders, and the recombinant protein can be used to detect these mutations and aid in diagnosis.
• Vaccine development: The recombinant protein has also been used as an antigen in vaccine development. By stimulating the immune system to produce antibodies against TRPM7/LTRPC7, researchers hope to develop a vaccine that can prevent or treat certain diseases associated with this protein.

Conclusion

The Recombinant Human TRPM7/LTRPC7 Protein is a versatile and important protein in the field of molecular biology and bio