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Recombinant Monkeypox virus/MPXV A35R Protein

Reference: PX-P6034-100
size

100ug

Brand

ProteoGenix

Product type

Recombinant Proteins

Host Species

Mammalian cells

Applications

Elisa, WB

Product nameRecombinant Monkeypox virus/MPXV A35R Protein
Origin speciesMonkeypox virus (strain Zaire-96-I-16) (MPX)
Expression systemEukaryotic expression
Molecular weight16.65 kDa
Protein delivered with Tag?C-terminal His Tag
Purity estimated>85% by SDS-PAGE
BufferPBS, pH7.5
Delivery conditionDry Ice
Delivery lead time in business days3-5 days if in stock; 3-5 weeks if production needed
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
Host speciesMammalian cells
Fragment TypeVal57-Thr181
Aliases /SynonymsMonkeypox A35R, clade 2
ReferencePX-P6034
NoteFor research use only.

SDS-PAGE For Recombinant Monkeypox virus/MPXV A35R Protein

Recombinant Monkeypox virus/MPXV A35R Protein, on SDS-PAGE under reducing condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

Anti-MPXV A35R Antibody (SAA0287) binds with A35R protein in ELISA assay

Immobilized Recombinant Monkeypox virus/MPXV A35R Protein (cat. No.PX-P6034) at 0.5µg/mL (100µL/well) can bind Monkeypox virus/MPXV A35R Monoclonal Antibody SAA0287 (cat. No.PTX18825) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450 giving an EC50 at 31.66M.

Publication

  • Daniel Stern, Rebecca Surtees, Fridolin Treindl et al. Differentiation between mpox infection and MVA immunization by a novel machine learning-supported serological multiplex assay, 09 August 2024, PREPRINT (Version 1) available at Research Square https://doi.org/10.21203/rs.3.rs-4822502/v1
  • Surtees R, Treindl F, Akhmedova S, et al. Machine learning-supported framework for the classification of mpox infection and MVA immunization from multiplexed serology data. Nature Communications. 2025 Dec;16(1):10943. DOI: 10.1038/s41467-025-66994-0. PMID: 41350540; PMCID: PMC12686480.https://doi.org/10.1038/s41467-025-66994-0

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