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Recombinant Monkeypox virus/MPXV M1R Protein

Reference: PX-P6056-100
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SDS-PAGE For Recombinant Monkeypox virus/MPXV M1R Protein
Anti-MPXV M1R Antibody(SAA0283) binds with M1R protein in ELISA assay
size

100ug
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QC & Validation Data2
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Product nameRecombinant Monkeypox virus/MPXV M1R Protein
Origin speciesMonkeypox virus (strain Zaire-96-I-16) (MPX)
Expression systemEukaryotic expression
Molecular weight22.35 kDa
Protein delivered with Tag?C-terminal His Tag
Purity estimated>85% by SDS-PAGE
BufferPBS, pH7.5
Delivery conditionDry Ice
Delivery lead time in business days3-5 days if in stock; 3-5 weeks if production needed
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
Host speciesMammalian cells
ApplicationsELISA,WB
Fragment TypeGly2-Gly183
Aliases /SynonymsM1R, Monkeypox virus/MPXV, clade 2, clade 1, clade 1b
ReferencePX-P6056
NoteFor research use only.
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SDS-PAGE For Recombinant Monkeypox virus/MPXV M1R Protein

Recombinant Monkeypox virus/MPXV M1R Protein, on SDS-PAGE under reducing conditions. The gel was stained overnight with Coomassie Blue.

Anti-MPXV M1R Antibody(SAA0283) binds with M1R protein in ELISA assay

Immobilized Recombinant Monkeypox virus/MPXV M1R Protein (cat. No. PX-P6056) at 0.5µg/mL (100µL/well) can bind Monkeypox virus/MPXV M1R SAA0283 Monoclonal Antibody SAA0283 (cat. No. PTX18821) in indirect ELISA with Goat Anti-Human IgG secondary antibody coupled with HRP measured by OD450 giving an EC50 at 136.5M.

Publication

Daniel Stern, Rebecca Surtees, Fridolin Treindl et al. Differentiation between mpox infection and MVA immunization by a novel machine learning-supported serological multiplex assay, 09 August 2024, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-4822502/v1]

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