Recombinant Mouse ANGPT2/Ang2 Protein, N-His

Description of Recombinant Mouse ANGPT2/Ang2 Protein, N-His

Introduction

Recombinant Mouse ANGPT2/Ang2 Protein is a genetically engineered version of the naturally occurring protein ANGPT2, also known as angiopoietin-2. This protein plays a crucial role in regulating blood vessel formation and maintenance, making it a valuable tool in various scientific and medical applications. In this article, we will explore the structure, activity, and applications of this recombinant protein.

Structure of Recombinant Mouse ANGPT2/Ang2 Protein

The recombinant version of ANGPT2 is produced by inserting the gene encoding for the protein into a host organism, typically a bacterial or mammalian cell. This allows for large-scale production of the protein for research and therapeutic purposes. The resulting protein has the same amino acid sequence as the natural ANGPT2, but may have slight differences in post-translational modifications.

The protein is composed of 496 amino acids and has a molecular weight of approximately 57 kDa. It consists of an N-terminal coiled-coil domain, a central fibrinogen-like domain, and a C-terminal fibrinogen-like domain. These domains are essential for the protein’s function in regulating blood vessel growth and permeability.

Activity of Recombinant Mouse ANGPT2/Ang2 Protein

ANGPT2 is a key regulator of blood vessel development and maintenance. It acts as an antagonist to another protein, ANGPT1, which promotes blood vessel stability. ANGPT2 binds to the Tie2 receptor on endothelial cells, the cells that line the inside of blood vessels, and inhibits the signaling pathway activated by ANGPT1. This leads to destabilization of blood vessels and promotes angiogenesis, the formation of new blood vessels.

In addition to its role in angiogenesis, ANGPT2 also plays a role in inflammation and tissue repair. It has been shown to promote the migration of immune cells to sites of injury and infection, aiding in the healing process.

Applications of Recombinant Mouse ANGPT2/Ang2 Protein

The recombinant version of ANGPT2 has a wide range of applications in scientific research and potential therapeutic use. Some of the major applications include:

Angiogenesis studies

As ANGPT2 is a key regulator of angiogenesis, the recombinant protein is commonly used in studies to understand the mechanisms of blood vessel formation and maintenance. It can be used to induce or inhibit angiogenesis in various in vitro and in vivo models, allowing researchers to investigate the role of ANGPT2 in different physiological and pathological conditions.

Cancer research

Angiogenesis is a crucial process in tumor growth and metastasis. The recombinant ANGPT2 protein has been used in cancer research to study the role of this protein in promoting tumor angiogenesis. It has also been investigated as a potential therapeutic target for anti-angiogenic cancer treatments.

Tissue engineering

Recombinant ANGPT2 has also been used in tissue engineering applications to promote the formation of new blood vessels in engineered tissues. This can aid in the development of functional and vascularized tissues for various medical applications.

Therapeutic potential

Due to its role in promoting angiogenesis and tissue repair, ANGPT2 has been investigated as a potential therapeutic agent for various diseases. It has shown promising results in preclinical studies for the treatment of cardiovascular diseases, wound healing, and inflammatory disorders.

Conclusion

Recombinant Mouse ANGPT2/Ang2 Protein is a valuable tool in scientific research and has potential therapeutic applications. Its structure and activity make it a crucial regulator of blood vessel formation and maintenance, with implications in various physiological and pathological processes. Further research and development of this protein may lead to new treatments for diseases related to angiogenesis and tissue repair.