Tiragolumab ELISA Kit

Description of Tiragolumab ELISA Kit

Tiragolumab ELISA Kit: Structure, Activity, and Application

Introduction

The Tiragolumab ELISA Kit is a highly sensitive and specific assay that is used for the detection and quantification of Tiragolumab, a novel immune checkpoint inhibitor, in various biological samples. This kit is designed for research use only and is not intended for diagnostic purposes.

Structure of Tiragolumab

Tiragolumab is a humanized monoclonal antibody that targets the immune checkpoint protein, TIGIT (T cell immunoreceptor with Ig and ITIM domains). It is composed of two heavy chains and two light chains, with a molecular weight of approximately 150 kDa. The antibody has a constant region (Fc) and a variable region (Fab) that specifically binds to TIGIT.

Activity of Tiragolumab

Tiragolumab works by blocking the interaction between TIGIT and its ligands, CD155 and CD112, which are expressed on tumor cells and immune cells. This interaction inhibits the activation and proliferation of T cells, leading to immune suppression and tumor growth. By blocking this interaction, Tiragolumab enhances T cell activation and promotes anti-tumor immune responses.

Application of Tiragolumab ELISA Kit

The Tiragolumab ELISA Kit is a valuable tool for researchers studying the role of TIGIT in cancer and developing novel therapies targeting this immune checkpoint. The kit can be used to measure the levels of Tiragolumab in various biological samples, such as serum, plasma, and cell culture supernatants. This allows for the evaluation of Tiragolumab pharmacokinetics and pharmacodynamics in preclinical and clinical studies.

Quantification of Tiragolumab in Serum and Plasma

The Tiragolumab ELISA Kit utilizes a competitive ELISA method, where Tiragolumab in the sample competes with a fixed amount of biotinylated Tiragolumab for binding to a TIGIT-coated plate. After washing away unbound components, a streptavidin-HRP conjugate is added to the plate, followed by the addition of a substrate solution. The intensity of the color produced is inversely proportional to the concentration of Tiragolumab in the sample, allowing for the quantification of Tiragolumab levels in serum and plasma samples.

Detection of Tiragolumab in Cell Culture Supernatants

The Tiragolumab ELISA Kit can also be used to measure the levels of Tiragolumab in cell culture supernatants. This is useful for monitoring the production of Tiragolumab in cell lines or in vitro studies, as well as for evaluating the binding affinity of different Tiragolumab variants.

Advantages of Tiragolumab ELISA Kit

The Tiragolumab ELISA Kit offers several advantages over other methods of quantifying Tiragolumab, such as Western blotting or flow cytometry. It is highly sensitive, with a detection limit of 0.1 ng/mL, and has a wide dynamic range of 0.5-100 ng/mL. The kit also has a short assay time of only 2 hours, making it a quick and efficient method for measuring Tiragolumab levels.

Conclusion

The Tiragolumab ELISA Kit is a reliable and sensitive assay for the detection and quantification of Tiragolumab, a promising immune checkpoint inhibitor. Its structure, activity, and application make it a valuable tool for researchers studying TIGIT and developing novel cancer therapies. With its high sensitivity and efficiency, this kit can contribute to the advancement of immunotherapy and ultimately improve patient outcomes.

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