Urelumab ELISA Kit

Description of Urelumab ELISA Kit

Introduction

Urelumab is a fully human monoclonal antibody that targets the immune checkpoint receptor, CD137, also known as 4-1BB. This receptor is expressed on the surface of activated T cells and plays a crucial role in regulating immune responses. Urelumab has shown promising results in clinical trials as a potential immunotherapy for various cancers, making it an important therapeutic target in the field of oncology.

Structure of Urelumab

Urelumab is a 150 kDa immunoglobulin G (IgG) antibody with a molecular weight of approximately 150 kDa. It is composed of two heavy chains and two light chains, connected by disulfide bonds. The heavy chain consists of four constant domains (CH1, CH2, CH3, and CH4) and one variable domain (VH), while the light chain contains one constant domain (CL) and one variable domain (VL). The variable domains are responsible for binding to the CD137 receptor, while the constant domains provide stability and effector functions.

Mechanism of Action

Urelumab works by binding to the CD137 receptor on activated T cells, leading to its activation and proliferation. This results in the expansion of T cells, which are important for mounting an effective immune response against cancer cells. Additionally, Urelumab can also induce the production of cytokines, such as interferon-gamma and tumor necrosis factor-alpha, which further enhance the anti-tumor activity of T cells.

Application of Urelumab ELISA Kit

The Urelumab ELISA Kit is a highly sensitive and specific tool for the quantitative detection of Urelumab in various biological samples, such as serum, plasma, and cell culture supernatants. This kit utilizes the sandwich enzyme-linked immunosorbent assay (ELISA) technique, where Urelumab is captured by a specific antibody coated on the microplate wells and then detected by a second antibody labeled with an enzyme. The amount of Urelumab present in the sample is directly proportional to the intensity of the color signal, which can be measured using a spectrophotometer.

Advantages of Urelumab ELISA Kit

• High sensitivity: The Urelumab ELISA Kit has a detection limit of 0.1 ng/mL, making it suitable for measuring low levels of Urelumab in biological samples.
• High specificity: The kit utilizes highly specific antibodies, ensuring minimal cross-reactivity with other proteins in the sample.
• Convenient and quick: The ELISA procedure is relatively simple and can be completed within a few hours, making it a time-efficient method for Urelumab detection.
• Cost-effective: The Urelumab ELISA Kit is a cost-effective alternative to other methods, such as Western blotting or flow cytometry, for Urelumab quantification.

Clinical Significance

The Urelumab ELISA Kit has significant clinical implications, especially in the field of cancer immunotherapy. It can be used to monitor the pharmacokinetics of Urelumab in patients receiving treatment, thereby aiding in dose optimization. Additionally, the kit can also be used to assess the presence of Urelumab in patient samples, which can help in predicting the response to therapy and monitoring treatment efficacy.

Conclusion

The Urelumab ELISA Kit is a valuable tool for the detection and quantification of Urelumab, a promising immunotherapy for cancer. Its high sensitivity, specificity, and convenience make it a preferred method for Urelumab analysis. With the increasing interest in Urelumab as a therapeutic target, the Urelum