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View ProductsGet a high yield protein expression in a eukaryotic system at affordable price! Trust our experts to optimize all the parameters and skyrocket the yield of your insect cell expression!
BACULOVIRUS EXPRESSION SERVICES
Choose the strongest expertise in protein expression!
- 1500+ protein expression
- 20 years of expertise
- From gene synthesis to protein expression
DATA REPORT
Get free access to genuine, representative data
from a Baculovirus/Insect cells Expression
& purification test project
An ideal example of the insights you can expect from this type of report
This comprehensive report includes:
- A full set of detailed data
- Advanced tests and results
- Key insights at every step of the process
Why choose ProteoGenix for your
baculovirus protein expression?
Integrated solutions
We propose a wide variety of cell lines and expression vectors to optimize your insect cell protein expression.
Unrivalled protein expression expertise
We routinely perform protein expression since 2003. 1500+ proteins expressed so far.
Optimized protocols
Our baculovirus protein expression service includes optimization of several parameters such as choice of insect cell line, multiplicity of infection and incubation time.
Integrated solutions
Choose a unique partner from gene synthesis to protein expression.
Codon optimization
Benefit from ProteoGenix’s gene synthesis expertise to get an optimized gene for insect cell expression.
PhD account managers
At ProteoGenix, you benefit immediately from the expertise of a PhD account manager!
Our insect cell protein expression process
Expression vector construction (optional)
- Gene design including codon optimization
- Gene Synthesis
- Subcloning in an expression vector
Virus production and amplification
- Transformation of the plasmid in competent E. coli cells to generate the Bacmid DNA
- Transfection of insect cells with the recombinant Bacmid
- Generation of the P1 Baculovirus stock
- Baculovirus amplification(P2 stock)
- Determination of virus titer
Small scale protein expression
- Transfection and selection of yeast clones
- Determination of top expressing clones
- Expression Optimization
- SDS/page + WB if necessary
- Purification tests
Protein expression scale up and purification
- Protein expression and purification with optimized conditions previously determined.
Our insect cell expression workflow
| Step | Content | Timeline | Deliverables |
|---|---|---|---|
| Virus production and amplification |
|
3 to 4 weeks |
|
| Protein expression scale up |
|
2 weeks |
|
| Virus production and amplification |
|
3 to 4 weeks |
|
