Antibody Development

Does your research, therapeutic, or diagnostic project require polyclonal or monoclonal antibody development expertise? Our custom antibody development services are a comprehensive one-stop solution, that will seamlessly guide you through every stage of the custom antibody generation process, from discovery to production, and any necessary antibody modifications in between. With over 6000 antibodies developed since our establishment in 2003, you can be sure of entrusting your project to an experienced team. The best part? Our custom antibody development process just got up to 50% faster using our cutting-edge RocketAbs platform. We stay in constant communication with your team and empower you to customize the project precisely to your requirements using our comprehensive list of antibody services. Let our team become an extension of your team. Contact us today for a free quote.

About Us – An antibody development company since 2003

Founded in 2003, ProteoGenix began developing diagnostic assays and biotherapeutics for novel protein identification. Naturally, excelling in the protein identification space involved developing high-quality antibodies to “visualize” our client’s protein of interest. However, ProteoGenix soon discovered it could produce novel antibodies in-house that significantly outperformed other custom antibody development companies on the market. So we wondered, why not make high-performing custom antibodies for the rest of the world?
That’s exactly what we did.

Twenty years later ProteoGenix has become a leader in the antibody production space. With 3 antibodies in clinical use and 30+ antibodies in preclinical and clinical trials, ProteoGenix established itself as a leader in the antibodies development industry.

ProteoGenix as an extension of your existing team

Let’s face it. Developing a custom antibody requires a lot of expertise, skill, and hard work. One wrong step and you can lose countless hours of time to an unforeseen error.
Developing your own antibodies in-house seems logical until you do the math. In reality, it’s cheaper to onboard our antibody experts at ProteoGenix and allow them to develop the antibody for you. After all, it’s what we are good at.

Not only will you save money in the short run, but you’ll also obtain long-term value because we stand behind the quality of each antibody we deliver.
Our antibody experts also stay in constant communication with you throughout each phase of the project. If there are any concerns along the way you will be the first to know what they are. We will consult with you and provide our expert opinion, but ultimately the decision to proceed with the project or terminate is yours. These checkpoints keep our customers informed and provide risky projects with a layer of monetary protection.

What is antibody development?

Antibody development is the process of developing custom antibodies that can recognize and bind to specific target molecules, known as antigens.
The process of antibody development typically involves the following steps:

1. Immunization: Animals or individuals are exposed to the target antigen through immunization. This exposure triggers the immune system to produce antibodies against the antigen.
2. Antibody Production: The immune system produces a diverse pool of antibodies that recognize different parts (epitopes) of the antigen.
3. Antibody Isolation: Blood samples are collected from the immunized individuals or animals. The antibodies present in the serum (polyclonal antibodies) or produced by specific B cells (monoclonal antibodies) are isolated.
4. Antibody Characterization: The isolated antibodies are then purified and characterized to assess their specificity, affinity, and other properties.
5. Antibody Application: The developed antibodies can be used for various applications, such as research, diagnostics, or therapeutics.

• Research, antibodies help scientists study and understand biological processes.
• Diagnostics, antibodies are used to detect specific molecules in patient samples to aid in disease diagnosis.
• Therapeutics, antibodies are used to treat diseases, including cancer, autoimmune disorders, and infectious diseases.

Custom antibody development is a critical process that has revolutionized medical research and healthcare, providing powerful tools to study diseases, identify biomarkers, and develop targeted therapies. The development of specific and reliable antibodies is a cornerstone of many scientific advancements, contributing to our understanding of biology and improving human health.

How are antibodies developed?

Polyclonal Antibody Development: In this approach, animals are immunized with the antigen causing the immune system of the animal to respond by producing a diverse pool of antibodies targeting various epitopes on the antigen. Blood is collected from the immunized animal, and the serum containing a mixture of antibodies is isolated.

Polyclonal antibodies provide broad recognition of the antigen, making them suitable for various applications in research and diagnostics.

Monoclonal Antibody Development: This process aims to produce highly specific antibodies that target a single epitope (a specific region) on a particular antigen. Unlike polyclonal antibodies which are a mixture of different antibodies targeting various epitopes, monoclonal antibodies are derived from a single B cell clone, resulting in a homogenous population of antibodies with identical binding properties.

ProteoGenix can develop your custom monoclonal antibodies in a variety of ways. Below is a brief description of the benefits and features of each antibodies development method.

• Antibody Phage Display: A library of antibody genes is fused to the gene sequence of a bacteriophage coat protein causing the antibody to be displayed on the surface of bacteriophages. The phage library is then screened for binding to the target antigen in vitro. The phages displaying the desired antibodies are selected, and the genes encoding these antibodies are isolated for further production (antibody genes are inside the bacteriophage). Phage display offers the advantage of identifying antibodies with high affinity and specificity for the target antigen.
• Single B Cell Isolation: This technique involves isolating individual B cells from immunized animals or individuals based on their ability to produce antibodies against the target antigen. These selected B cells are then used to generate monoclonal antibodies, allowing researchers to specifically target B cells that produce the desired antibodies.
• Hybridoma Cell Line Generation: This classical method involves immunizing an animal with the target antigen to elicit an immune response. B cells producing the desired antibodies are then isolated from the immunized animal. These B cells are fused with immortal myeloma cells to create hybridoma cells. The hybridomas can continuously produce the desired monoclonal antibodies with high specificity and reproducibility. Hybridoma technology is well-established and has been widely used for the development of monoclonal antibodies.

How much does it cost to develop an antibody?

The cost of antibody development varies ranging from approximately 310€ for our basic custom polyclonal antibody conjugation services to several tens of thousands of dollars for complex custom monoclonal antibody (mAb) development projects.

Rest assured, we provide strong guarantees and implement multiple go/no-go checkpoints that are built into large intricate projects. This ensures the security of your investment. Our diverse pricing options allow researchers and institutions to find the right solution tailored to their specific needs and budget while maintaining the highest standards of quality and reliability.

How long does it take to develop an antibody

The duration of antibody development can vary depending on the complexity of the project and the chosen techniques. For instance, polyclonal antibodies are delivered in one month while more intricate monoclonal antibody methods, such as antibody phage display, typically take around 7 weeks.

Factors such as the time required to secure the antigen, screening, antibody characterization, quality control, and project complexity contribute to the timeline. Despite the varying timeframes, our dedicated team ensures efficient progress while maintaining the highest standards of quality, ensuring that researchers receive their developed antibodies within reasonable timeframes.