Custom Hybridoma Development

Guaranteed performance for your application

>0%

Success rate

Days Fast-Track Protocol

Clones successfully developed

Guaranteed Applications

Hybridoma technology is ideal for generating high-affinity monoclonal antibodies with stable long-term expression, perfectly suited for ELISA, sandwich ELISA, Western blot, flow cytometry, immunofluorescence, immunoprecipitation, and immunohistochemistry applications. It complements other discovery methods such as phage display and single B cell screening, offering an efficient route to validated, application-ready antibodies.

Get Your Hybridoma in 45 Days, Guaranteed to Work for Your Application

We guarantee the functionality of your antibody in the application of your choice, whether ELISA, sandwich ELISA, Western blot, flow cytometry, immunofluorescence, immunoprecipitation, or immunohistochemistry.
You only pay the full price once the hybridoma has been successfully validated in your own laboratory under your specific conditions.

Start your Custom Hybridoma project

Your Hybridoma Development Journey with Full Visibility and Control

At ProteoGenix, we develop real partnership with our clients. That is why we implemented multiple GO/NO GO checkpoints along our process. Our clients always remain in control of their project and can make informed decisions every step of the way.

Antigen Design

Define immunization strategy
Perform peptide synthesis, gene synthesis, and protein production or opt for DNA immunization

GO/NO GO

Immunization

Immunize 5 mice using a proprietary protocol to generate a robust immune response

GO/NO GO

Cell Fusion

Collect splenocytes from 2 mice
Perform cell fusion with a myeloma cell line to create hybridomas

GO/NO GO

Hybridoma Selection And Screening

Select hybrid cells using HAT selection
Expand and screen monoclones by ELISA or in the target application for the highest quality antibodies

GO/NO GO

Isolation And Selection Of The Best Monoclones

Isolate monoclones through limiting dilution
Expand and screen monoclones by ELISA or in the target application for the highest quality antibodies

A Custom Hybridoma Development service
adapted to your final use

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Therapeutic Applications

ProteoGenix leverages over 20 years of expertise in hybridoma technology to develop potent antibody therapies with unmatched cost efficiency.
Our custom hybridoma development services produce antibodies with superior affinity, epitope specificity, and stability, ideal for challenging antigens.
With 30+ antibodies in trials and 3 already on the market, we’re your trusted partner from lab to clinic.

Start your Therapeutic Project

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Diagnostic & Research Applications

Start creating next-generation antibody-based diagnostics with ProteoGenix’s risk-free hybridoma production.
Our custom hybridoma monoclonal antibody services guarantee optimal performance in any diagnostic application, including flow cytometry, sandwich ELISA, IHC, IF, IP, and WB. With ten development packages and swift access to downstream services such as antibody conjugation and custom ELISA development, we ensure your challenging projects move quickly from concept to market. Our approach delivers high-affinity antibodies tailored to your specific assay conditions and sample types, empowering your diagnostic and research tools with unparalleled precision and reliability.

Start your Diagnostic/Research Project

DATA REPORT

Get free access to genuine, representative data
from a Hybridoma Development project

An ideal example of the insights you can expect from this type of report

This comprehensive report includes:

A full set of detailed data
Advanced tests and results
Key insights at every step of the process

Download the Data Report

Testimonials from our clients

Ulm Institute of Immunology, Germany

Dr. rer. nat. Rudolf Übelhart, Project Leader

“We collaborated on a project for generating a monoclonal antibody that recognizes a one-amino-acid mutation in a cancer-associated cell-surface protein. Although the project was technically challenging, we succeeded in generating a hybridoma clone that produced the desired antibody. During the course of the project, we found additional clones that recognized adjacent epitopes and ProteoGenix proceeded to perform additional subclonings in order to ensure the delivery of clones that work perfectly. In summary, ProteoGenix always strived to ensure maximal product quality and customer satisfaction, and the account Manager provided prompt and competent support at all times.”

Bangor University, UK

Dr. David Pryce, Lecturer in Biomedical Sciences (Immunology)

“I requested ProteoGenix’s services for the generation of an anti-peptide monoclonal antibody to be used in my research. I required the antibody primarily for western blotting and immunoprecipitation experiments. Although the peptide antigen proved challenging in terms of immunogenicity, ProteoGenix was able to propose a range of customised strategies to overcome production issues encountered and finally succeeded in generating not only one but three antibodies of interest, thus offering me extra options in terms of final choice and use. During the whole project, Proteogenix strived to ensure maximal quality and to adapt to my needs, and my Account Manager always provided prompt and helpful services and solutions. I can highly commend both the level of expertise and the service I received.”

Lille University, France

Dr. Karine Lecointe, Researcher

“We contacted ProteoGenix for a project concerning the detection of a phytopathogen. ProteoGenix helped us to the production of a specific antigen and the development of a monoclonal antibody to detect it by ELISA test and western blot. We appreciated the contact, the reactivity and professionalism of ProteoGenix for the good running of the project. We are satisfied with the services of the company.”

University of Birmingham, UK

Dr Julie Rayes, Associate Professor

“I contacted ProteoGenix regarding the generation of monoclonal antibodies for the detection of a chemokine. ProteoGenix generated for us multiple monoclonal antibodies to detect the chemokine by ELISA, western blot and to test these antibodies in functional assays. They provided an excellent service, with regular updates, quick response to our queries and an excellent delivery of the product. I recommend their service for their efficacy and professionalism.”

Lund University, Sweden

Dr Anna Blom, Professor

“I highly recommend ProteoGenix for their fast and efficient services, providing top-quality antibodies suitable for diverse applications. Their exceptional communication, quick turnaround time, and reasonable pricing make them an excellent choice even for non-standard projects.”

Canopy Immuno-Therapeutics, Israel

Dr Galit Denkberg, Vice-President Discovery

“We contacted ProteoGenix to develop repertoires of specific antibodies against three antigens sharing high homology. As determined by ELISA, ProteoGenix developed several specific antibodies that exhibited no cross-reactivity with the other antigens. We appreciated their proactive approach to increase our chances of success (e.g., performing fusions at their own risks). We are satisfied with the services of the company.”

T3 Pharmaceuticals AG, Switzerland

Dr. Simon Marlaire, Senior researcher

“We needed to develop two monoclonal antibodies for Western-Blot, from antigen synthesis to hybridoma cell line and purified antibody delivery. ProteoGenix met all our expectations with efficient communication, satisfying deadlines, and successful production of specific and functional antibodies. For future antibody production, ProteoGenix will remain our preferred partner.“

FAQ

Our hybridoma antibody production services are highly versatile, accommodating a wide range of antigens including cells, peptides, small molecules, proteins, DNA, VLPs, and nanodiscs.
Through peptide synthesis and DNA immunization, we tailor each project to ensure optimal antigen presentation and immune response.
We usually require only a few milligrams of antigen with at least 90% purity, and if needed, ProteoGenix can produce and design the antigen for you, whether in its native conformation for therapeutic contexts or denatured form for analytical assays such as Western Blot.
By prioritizing antigen design and offering a comprehensive evaluation phase, we develop biologically relevant antibodies precisely aligned with your project’s objectives.
Initial screening employs ELISA, complemented by flow cytometry and other assays to ensure antibodies meet application-specific requirements. This meticulous screening process guarantees our antibodies’ optimal performance across diagnostic and therapeutic applications. We also send our clients clone materials for them to test and maximize chances of success.
Yes, we can. For difficult membrane proteins, we employ nanodiscs or virus-like particles (VLPs) to enhance antigen presentation. For low-immunogenic targets, we use specific adjuvants or optimized injection routes, such as spleen injection, to maximize the immune response.
The duration of our custom hybridoma generation process varies, but we aim for efficiency with positive clones available within 45 days, thanks to our fast-track protocol. The entire project typically lasts around 2 months, including time for thorough validation testing.
Clients retain full ownership of the intellectual property (IP) for all custom antibodies and hybridomas developed through our service. ProteoGenix does not claim any rights over the generated materials, ensuring that clients have 100% control and exclusivity over their results.
After hybridoma development, we offer a complete range of antibody engineering and optimization options to support your next steps. These include humanization, sequencing, recombinant antibody production, hybridoma cell bank creation, ELISA development, ADC and bispecific antibody generation, as well as developability improvement to ensure your antibody performs optimally in its intended application.
We ensure transparent and regular communication throughout the project. Clients receive email updates at the end of each key step and calls at major milestones to review progress. At project completion, a comprehensive final report summarizing all results and experimental details is provided.
In the rare event of a project failure (less than 5% of cases), our guarantee policy ensures a refund, providing complete peace of mind. For low-response immunizations, we optimize the strategy using higher antigen doses, alternative adjuvants, or specific injection routes such as spleen injection, which typically results in a successful immune response.

More antibody discovery and expression platforms

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Custom Hybridoma Development

Guaranteed performance for your application

Get Your Hybridoma in 45 Days, Guaranteed to Work for Your Application