Recombinant Human COPS8 Protein, N-His

Description of Recombinant Human COPS8 Protein, N-His

Introduction

Recombinant Human COPS8 Protein, also known as COP9 Signalosome Complex Subunit 8, is a protein that plays a crucial role in regulating protein degradation and cell cycle progression. This protein is produced through recombinant technology, making it a valuable tool in scientific research and potential therapeutic applications. In this article, we will discuss the structure, activity, and potential applications of Recombinant Human COPS8 Protein.

Structure of Recombinant Human COPS8 Protein

Recombinant Human COPS8 Protein is a 25 kDa protein that consists of 228 amino acids. It is composed of a highly conserved N-terminal domain and a less conserved C-terminal domain. The N-terminal domain contains a coiled-coil region, which is involved in protein-protein interactions, while the C-terminal domain contains a JAB1/MPN/Mov34 metalloenzyme (JAMM) motif, which is responsible for its enzymatic activity.

Activity of Recombinant Human COPS8 Protein

Recombinant Human COPS8 Protein is a subunit of the COP9 signalosome (CSN) complex, which is a highly conserved protein complex that regulates the activity of cullin-RING E3 ubiquitin ligases (CRLs). CRLs are responsible for targeting specific proteins for degradation through the ubiquitin-proteasome pathway. The CSN complex acts as a deneddylase, removing the NEDD8 modification from cullins, thereby regulating their activity and preventing premature degradation of target proteins.

Recombinant Human COPS8 Protein specifically binds to cullin-2 and cullin-5, two members of the CRL family, and enhances their deneddylation activity. This activity is essential for proper cell cycle progression and cellular homeostasis. In addition, Recombinant Human COPS8 Protein has been shown to interact with various other proteins, including transcription factors, kinases, and cell cycle regulators, suggesting its involvement in a wide range of cellular processes.

Applications of Recombinant Human COPS8 Protein

Recombinant Human COPS8 Protein has various applications in both basic research and potential therapeutic interventions. One of its main uses is in studying the function of the CSN complex and its role in regulating protein degradation. By using recombinant COPS8 protein, researchers can manipulate the activity of the CSN complex and study its effects on various cellular processes.

Furthermore, Recombinant Human COPS8 Protein has been implicated in various diseases, including cancer and neurodegenerative disorders. Its involvement in regulating the activity of CRLs makes it a potential target for therapeutic interventions. By targeting Recombinant Human COPS8 Protein, researchers can potentially modulate the activity of CRLs and prevent the degradation of specific proteins that contribute to disease progression.

In addition, Recombinant Human COPS8 Protein can also be used as an antigen for antibody production. Its high expression in various tissues and its involvement in multiple cellular processes make it an attractive target for generating specific antibodies for research and diagnostic purposes.

Conclusion

Recombinant Human COPS8 Protein is a crucial component of the CSN complex, which plays a vital role in regulating protein degradation and cell cycle progression. Its structure, activity, and potential applications make it a valuable tool in scientific research and a potential target for therapeutic interventions. As our understanding of this protein continues to grow, so does its potential for further advancements in various fields of study.