Recombinant Human KCNA3 Protein, N-His

Description of Recombinant Human KCNA3 Protein, N-His

Title: Introduction to Recombinant Human KCNA3 Protein

Recombinant Human KCNA3 Protein, also known as the voltage-gated potassium channel subfamily A member 3, is a protein that plays a crucial role in regulating the electrical activity of cells. This protein is encoded by the KCNA3 gene and is found in various tissues, including the brain, heart, and skeletal muscles. In this article, we will explore the structure, activity, and applications of Recombinant Human KCNA3 Protein.

Title: Structure of Recombinant Human KCNA3 Protein

Recombinant Human KCNA3 Protein is a transmembrane protein that consists of six subunits, each with four transmembrane domains. These domains form a pore that allows the flow of potassium ions across the cell membrane. The structure of this protein is highly conserved among species, with a high degree of similarity to other potassium channels.

Title: Activity of Recombinant Human KCNA3 Protein

The primary function of Recombinant Human KCNA3 Protein is to regulate the flow of potassium ions across the cell membrane. This activity is essential for maintaining the resting membrane potential of cells, which is crucial for various physiological processes such as muscle contraction and nerve signaling. Recombinant Human KCNA3 Protein is also involved in the repolarization of neurons, which is necessary for proper functioning of the nervous system.

Title: Mechanism of Action

The activity of Recombinant Human KCNA3 Protein is regulated by various mechanisms, including voltage and ligand-gated channels. When a cell is at rest, the channel remains closed, preventing the flow of potassium ions. However, when the cell is stimulated, the channel opens, allowing potassium ions to flow out of the cell. This results in the repolarization of the cell, restoring the resting membrane potential.

Title: Applications of Recombinant Human KCNA3 Protein

Recombinant Human KCNA3 Protein has various applications in both research and clinical settings. In research, this protein is used to study the role of potassium channels in various physiological processes. It is also used to investigate the effects of mutations in the KCNA3 gene, which have been associated with various neurological disorders.

In the clinical setting, Recombinant Human KCNA3 Protein is used as an antigen for diagnostic tests for autoimmune diseases such as multiple sclerosis and myasthenia gravis. These diseases are characterized by the production of autoantibodies against potassium channels, including KCNA3. By using Recombinant Human KCNA3 Protein as an antigen, these autoantibodies can be detected and used for diagnosis and monitoring of these diseases.

Title: Production of Recombinant Human KCNA3 Protein

Recombinant Human KCNA3 Protein is produced using recombinant DNA technology. The KCNA3 gene is cloned into a suitable expression vector and then introduced into host cells, such as bacteria or mammalian cells. The host cells then produce large quantities of the protein, which can be purified and used for various applications.

Title: Conclusion

In conclusion, Recombinant Human KCNA3 Protein is a crucial protein involved in regulating the electrical activity of cells. Its structure and activity make it an essential component in various physiological processes, and its applications in research and clinical settings make it a valuable tool in understanding and diagnosing diseases. With the advancement of recombinant DNA technology, the production of this protein has become more accessible, allowing for further research and development in this field.