Recombinant Human MYOG Protein, N-His-SUMO

Description of Recombinant Human MYOG Protein, N-His-SUMO

Title: Introduction to Recombinant Human MYOG Protein

Recombinant Human MYOG Protein, also known as Myogenin, is a key regulatory protein involved in muscle development and regeneration. It belongs to the Myogenic Regulatory Factor (MRF) family and plays a crucial role in skeletal muscle differentiation. In this article, we will explore the structure, activity and applications of Recombinant Human MYOG Protein.

Title: Structure of Recombinant Human MYOG Protein

Recombinant Human MYOG Protein is a 224 amino acid protein with a molecular weight of 25 kDa. It consists of a basic helix-loop-helix (bHLH) domain, a leucine zipper (LZ) domain, and a transcription activation domain (TAD). The bHLH domain is responsible for DNA binding and dimerization, while the LZ domain mediates protein-protein interactions. The TAD is essential for the transcriptional activity of MYOG.

Title: Activity of Recombinant Human MYOG Protein

MYOG is a transcription factor that regulates the expression of genes involved in muscle differentiation. It binds to specific DNA sequences known as E-boxes and recruits other transcription factors to activate gene expression. MYOG also interacts with chromatin remodeling complexes to facilitate the formation of muscle-specific chromatin structures. This results in the activation of genes required for muscle development and regeneration.

Title: Applications of Recombinant Human MYOG Protein

1. Cell Differentiation Studies: Recombinant Human MYOG Protein is widely used in cell differentiation studies to understand the molecular mechanisms involved in muscle development. It can be added to cell culture media to induce myogenic differentiation in myoblasts, leading to the formation of muscle-like cells.

2. Gene Therapy: MYOG has been identified as a potential therapeutic target for muscle disorders such as muscular dystrophy. Recombinant Human MYOG Protein can be used in gene therapy approaches to promote muscle regeneration and repair damaged muscle tissue.

3. Biomarker for Muscle Diseases: MYOG expression is elevated in various muscle diseases, making it a potential biomarker for disease diagnosis and progression. Recombinant Human MYOG Protein can be used in diagnostic tests to detect the levels of MYOG in patient samples.

4. Protein Production: Recombinant Human MYOG Protein can be produced in large quantities using recombinant DNA technology. It is used in the production of proteins for research and therapeutic purposes.

5. Vaccine Development: MYOG has been identified as an antigen in certain types of cancer, making it a potential target for vaccine development. Recombinant Human MYOG Protein can be used to trigger an immune response against cancer cells expressing MYOG.

Title: Conclusion

In conclusion, Recombinant Human MYOG Protein is a crucial protein involved in muscle development and regeneration. Its structure, activity, and applications make it a valuable tool for research and therapeutic purposes. With ongoing studies and advancements in technology, Recombinant Human MYOG Protein holds great potential for the treatment of muscle disorders and other diseases.