Recombinant Human PDCD6IP, N-GST

Description of Recombinant Human PDCD6IP, N-GST

Introduction

Recombinant Human PDCD6IP, also known as Programmed Cell Death 6 Interacting Protein (PDCD6IP), is a protein that plays a crucial role in several cellular processes, including apoptosis, cell proliferation, and intracellular signaling. This protein is encoded by the PDCD6IP gene, which is located on chromosome 5 in humans. Recombinant Human PDCD6IP is a synthetic version of the naturally occurring protein, produced through genetic engineering techniques. In this article, we will discuss the structure, activity, and applications of this recombinant protein.

Structure of Recombinant Human PDCD6IP

Recombinant Human PDCD6IP is a 39-kDa protein consisting of 344 amino acids. It contains a highly conserved N-terminal domain, a central coiled-coil domain, and a C-terminal domain. The N-terminal domain is responsible for the interaction with other proteins, while the coiled-coil domain is involved in protein-protein interactions and oligomerization. The C-terminal domain contains a calcium-binding EF-hand motif, which is important for the regulation of PDCD6IP activity.

Activity of Recombinant Human PDCD6IP

Recombinant Human PDCD6IP is a multifunctional protein that is involved in various cellular processes. One of its main functions is the regulation of apoptosis, or programmed cell death. It does so by interacting with the anti-apoptotic protein Bcl-2 and promoting its degradation, leading to the activation of pro-apoptotic proteins and initiation of the apoptotic pathway. PDCD6IP has also been shown to regulate cell proliferation by modulating the activity of the transcription factor c-Myc, which is involved in cell growth and division.

In addition, Recombinant Human PDCD6IP plays a role in intracellular signaling pathways. It acts as a scaffold protein, bringing together various signaling molecules and facilitating their interactions. This is important for the activation of downstream signaling pathways, such as the mitogen-activated protein kinase (MAPK) pathway, which is involved in cell growth and differentiation.

Applications of Recombinant Human PDCD6IP

Due to its diverse functions, Recombinant Human PDCD6IP has a wide range of applications in both basic research and clinical settings. One of its main uses is in the study of apoptosis and cell proliferation. By modulating PDCD6IP expression or activity, researchers can investigate the role of this protein in these processes and its potential as a therapeutic target.

Recombinant Human PDCD6IP has also been used in cancer research, as it has been shown to play a role in tumor growth and progression. In particular, its interaction with Bcl-2 has been linked to drug resistance in cancer cells. By studying the mechanisms of PDCD6IP-mediated apoptosis, researchers may be able to develop new strategies to overcome drug resistance and improve cancer treatment.

In addition, Recombinant Human PDCD6IP has potential applications in the development of diagnostic tools. As it is involved in various signaling pathways, its expression or activity levels may serve as biomarkers for certain diseases. For example, increased PDCD6IP expression has been linked to the development of Alzheimer’s disease, making it a potential biomarker for early detection and monitoring of this neurodegenerative disorder.

Conclusion

Recombinant Human PDCD6IP is a versatile protein with important roles in apoptosis, cell proliferation, and intracellular signaling. Its structure and activity have been extensively studied, and its potential applications in basic research and clinical settings are promising. As research on this protein continues, we may uncover new insights into its functions and potential therapeutic uses.