Recombinant Human PLEKHO1 Protein, N-His

Description of Recombinant Human PLEKHO1 Protein, N-His

Introduction

Recombinant proteins are proteins that are produced through genetic engineering techniques, where the gene for a specific protein is inserted into a host organism, such as bacteria or yeast, to produce large quantities of the desired protein. These proteins have become essential tools in various fields of research and medicine due to their high purity, specificity, and functionality. One such recombinant protein is the Recombinant Human PLEKHO1 Protein.

Structure of Recombinant Human PLEKHO1 Protein

PLEKHO1 (Pleckstrin homology domain-containing, family O member 1) is a protein that plays a crucial role in the regulation of cell adhesion and migration. The human PLEKHO1 gene is located on chromosome 1 and encodes a protein of 300 amino acids. The recombinant form of PLEKHO1 is produced by inserting the gene into a suitable expression vector and expressing it in a host organism. The resulting protein has a molecular weight of approximately 34 kDa and contains the pleckstrin homology (PH) domain, which is responsible for binding to phospholipids and regulating cellular signaling pathways.

Activity of Recombinant Human PLEKHO1 Protein

The main function of PLEKHO1 is to regulate cell migration and adhesion by interacting with other proteins and lipids. The PH domain of PLEKHO1 binds to phosphatidylinositol 4,5-bisphosphate (PIP2) and phosphatidylinositol 3,4,5-trisphosphate (PIP3), which are important signaling molecules involved in cell migration. This binding leads to the activation of downstream signaling pathways, such as the PI3K/Akt pathway, which promotes cell migration and invasion. PLEKHO1 also interacts with other proteins, such as integrins and focal adhesion kinase (FAK), to regulate cell adhesion and migration.

Applications of Recombinant Human PLEKHO1 Protein

Recombinant Human PLEKHO1 Protein has various applications in both research and medicine. It is commonly used in cell migration and adhesion studies, where its interaction with PIP2 and PIP3 can be studied to understand the underlying mechanisms of cell migration. PLEKHO1 is also being investigated as a potential therapeutic target for diseases involving abnormal cell migration, such as cancer and cardiovascular diseases. In addition, recombinant PLEKHO1 can be used as an antigen in the production of antibodies for research and diagnostic purposes.

Conclusion

Recombinant Human PLEKHO1 Protein is a valuable tool in scientific research and medicine. Its structure, activity, and applications make it a crucial protein in the study of cell migration and adhesion. With further research, PLEKHO1 may also prove to be a promising therapeutic target for diseases involving abnormal cell migration. The production and use of recombinant proteins, such as PLEKHO1, have greatly advanced our understanding of biological processes and have the potential to contribute to the development of new treatments for various diseases.