Recombinant Human RBM8A Protein, N-His-SUMO

Description of Recombinant Human RBM8A Protein, N-His-SUMO

Introduction

Recombinant Human RBM8A protein, also known as Y14, is a highly conserved protein that plays a crucial role in RNA metabolism and splicing. It is encoded by the RBM8A gene and is found in all eukaryotic organisms.

Structure of Recombinant Human RBM8A Protein

The RBM8A protein is composed of 130 amino acids and has a molecular weight of approximately 14.8 kDa. It contains a conserved RNA recognition motif (RRM) domain, which is responsible for its RNA binding activity. This domain is located at the N-terminus of the protein and is essential for its function in RNA splicing.

In addition to the RRM domain, the RBM8A protein also has a C-terminal domain that is involved in protein-protein interactions. This domain is required for the formation of the exon junction complex (EJC), which is a multiprotein complex involved in post-transcriptional gene regulation.

Activity of Recombinant Human RBM8A Protein

The main function of RBM8A protein is to regulate RNA splicing by binding to specific sequences in pre-mRNA molecules. It is a component of the EJC, which is deposited on mature mRNAs during splicing. The EJC plays a crucial role in mRNA localization, translation, and degradation.

RBM8A protein is also involved in nonsense-mediated mRNA decay (NMD), a process that degrades mRNAs containing premature stop codons. It is required for the recognition of these mRNAs by the NMD machinery and for their subsequent degradation.

Moreover, RBM8A protein has been shown to interact with other proteins involved in RNA metabolism, such as UPF3B and MAGOH. These interactions are essential for the proper functioning of the EJC and NMD processes.

Application of Recombinant Human RBM8A Protein

The recombinant form of RBM8A protein is widely used in various research applications. One of its main applications is in studying the role of RNA splicing and post-transcriptional gene regulation. Recombinant RBM8A protein can be used in in vitro splicing assays to investigate its binding to pre-mRNA and its role in splicing regulation.

Furthermore, recombinant RBM8A protein can be used to study the function of the EJC and NMD processes. It can be used to identify and characterize other proteins that interact with RBM8A and to understand their role in these processes.

In addition, recombinant RBM8A protein has potential therapeutic applications. Mutations in the RBM8A gene have been associated with a rare genetic disorder called Nager syndrome. Recombinant RBM8A protein can be used to study the effects of these mutations and to develop potential treatments for this disorder.

Conclusion

In summary, recombinant Human RBM8A protein is a crucial component of the EJC and plays a vital role in RNA splicing and post-transcriptional gene regulation. Its structure, activity, and application have been extensively studied, and it continues to be an essential protein in the field of RNA biology and related research areas.