Recombinant Human SIAH2 Protein, N-His

Description of Recombinant Human SIAH2 Protein, N-His

Introduction

Recombinant Human SIAH2 Protein is a highly versatile protein that has gained significant attention in the field of biomedical research. It is a recombinant protein, meaning it is produced through genetic engineering techniques, and is a crucial player in various cellular processes. In this article, we will explore the structure, activity, and application of this protein in detail.

Structure of Recombinant Human SIAH2 Protein

The SIAH2 gene encodes for the SIAH2 protein, which is a member of the E3 ubiquitin-protein ligase family. This protein is composed of 323 amino acids and has a molecular weight of approximately 39 kDa. It consists of three main domains: an N-terminal RING finger domain, a central SIAH domain, and a C-terminal substrate-binding domain.

The RING finger domain is responsible for the E3 ubiquitin ligase activity of SIAH2, while the SIAH domain is essential for its interaction with other proteins. The substrate-binding domain contains a conserved substrate-binding motif, which is responsible for binding to specific target proteins for ubiquitination and subsequent degradation.

Activity of Recombinant Human SIAH2 Protein

SIAH2 is a critical regulator of various cellular processes, including cell proliferation, differentiation, and apoptosis. Its main function is to target specific proteins for degradation by the ubiquitin-proteasome system. This process involves the attachment of ubiquitin molecules to the target protein, marking it for destruction by the proteasome.

Studies have shown that SIAH2 plays a crucial role in the regulation of the hypoxia response pathway. It targets hypoxia-inducible factor 1-alpha (HIF-1α) for degradation, thereby preventing the activation of genes involved in promoting cell survival under low oxygen conditions. Additionally, SIAH2 has been found to regulate the levels of several tumor suppressor proteins, such as p53 and PTEN, making it a potential target for cancer therapy.

Application of Recombinant Human SIAH2 Protein

Due to its diverse functions, SIAH2 has become a subject of interest in various fields of research. One of the major applications of recombinant SIAH2 protein is in drug discovery and development. As mentioned earlier, SIAH2 has been implicated in cancer progression, making it a potential target for anti-cancer drugs. Researchers are currently investigating the use of SIAH2 inhibitors as a novel approach for cancer treatment.

Another potential application of SIAH2 protein is in the field of regenerative medicine. Studies have shown that SIAH2 plays a critical role in the differentiation of stem cells into various cell types. This finding opens up the possibility of using SIAH2 protein to control and direct stem cell differentiation, which can have significant implications in tissue engineering and regenerative medicine.

Furthermore, SIAH2 has been linked to several neurodegenerative diseases, such as Alzheimer’s and Parkinson’s disease. It has been found to regulate the levels of proteins involved in these diseases, making it a potential therapeutic target.

Conclusion

In summary, Recombinant Human SIAH2 Protein is a highly versatile protein with a crucial role in various cellular processes. Its structure, activity, and application have been extensively studied, and it has shown great potential in the fields of drug discovery, regenerative medicine, and neurodegenerative diseases. Further research on this protein is essential for a better understanding of its functions and potential therapeutic applications.