Recombinant Mouse CD16/FcRIII Protein, N-His

Description of Recombinant Mouse CD16/FcRIII Protein, N-His

Introduction

Recombinant proteins have become an essential tool in biomedical research, drug development, and clinical applications. One such protein is the Recombinant Mouse CD16/FcRIII Protein, which has gained significant attention due to its structure, activity, and diverse applications. In this article, we will provide a detailed description of this protein, including its structure, function, and applications.

Structure of Recombinant Mouse CD16/FcRIII Protein

Recombinant Mouse CD16/FcRIII Protein is a glycoprotein that belongs to the immunoglobulin superfamily. It is composed of two subunits, the alpha and gamma chains, and is encoded by the Fcgr3 gene. The alpha chain contains two extracellular immunoglobulin domains, a transmembrane domain, and a short cytoplasmic tail. The gamma chain, on the other hand, contains a single extracellular immunoglobulin domain, a transmembrane domain, and a long cytoplasmic tail.

Activity of Recombinant Mouse CD16/FcRIII Protein

Recombinant Mouse CD16/FcRIII Protein is primarily known for its role in mediating antibody-dependent cell-mediated cytotoxicity (ADCC). This process involves the binding of the Fc region of an antibody to the Fc receptor on the surface of natural killer (NK) cells, leading to the activation of these cells and subsequent killing of target cells. CD16/FcRIII is the only Fc receptor expressed on the surface of NK cells, making it a crucial player in ADCC.

Apart from its role in ADCC, Recombinant Mouse CD16/FcRIII Protein also plays a role in immune modulation. It has been shown to regulate the production of cytokines, such as interferon-gamma and tumor necrosis factor-alpha, by NK cells. This modulation of cytokine production is essential for the activation and regulation of immune responses.

Applications of Recombinant Mouse CD16/FcRIII Protein

Recombinant Mouse CD16/FcRIII Protein has a wide range of applications in both basic research and clinical settings. Some of the key applications of this protein include:

1. Study of immune cell function: The use of recombinant CD16/FcRIII protein in in vitro studies has provided valuable insights into the function of NK cells and their role in immune responses. It has also been used to investigate the mechanisms of ADCC and the regulation of cytokine production by NK cells.

2. Development of therapeutic antibodies: The interaction between CD16/FcRIII and the Fc region of antibodies is crucial for the efficacy of therapeutic antibodies. Recombinant CD16/FcRIII protein has been used to screen and select antibodies with high affinity for this receptor, thus aiding in the development of more effective therapeutics.

3. Diagnosis and monitoring of diseases: CD16/FcRIII expression on NK cells has been linked to various diseases, including cancer and autoimmune disorders. The use of recombinant CD16/FcRIII protein in flow cytometry has enabled the identification and monitoring of NK cells in these diseases, providing valuable diagnostic and prognostic information.

4. Immunotherapy: The ability of CD16/FcRIII to activate NK cells and induce ADCC has made it a promising target for immunotherapy. Recombinant CD16/FcRIII protein has been used in the development of novel immunotherapeutic approaches, such as bispecific antibodies, that can activate NK cells and target cancer cells.

Conclusion

In conclusion, Recombinant Mouse CD16/FcRIII Protein is a glycoprotein that plays a critical role in immune modulation and ADCC. Its structure, activity, and diverse applications make it a valuable tool in biomedical research and clinical applications. Further studies on this protein may lead to the development of novel immunotherapeutic approaches and improved understanding of immune cell function.