Recombinant Mouse GSDMD/Gasdermin-D Protein, N-His-SUMO

Description of Recombinant Mouse GSDMD/Gasdermin-D Protein, N-His-SUMO

Introduction

Recombinant Mouse GSDMD/Gasdermin-D Protein is a highly sought-after protein in the field of immunology and cell biology. It is a member of the Gasdermin family, which consists of six proteins that play important roles in various cellular processes such as cell death, inflammation, and cell differentiation. The recombinant form of this protein has been extensively studied and has shown great potential in various applications, making it a valuable tool for researchers.

Structure of Recombinant Mouse GSDMD/Gasdermin-D Protein

The recombinant form of GSDMD/Gasdermin-D protein is a 55 kDa protein composed of 484 amino acids. It consists of an N-terminal domain (NTD), a central domain (CD), and a C-terminal domain (CTD). The NTD contains a conserved domain known as the gasdermin domain, which is responsible for the pore-forming activity of the protein. The CD is highly variable among different members of the Gasdermin family and is involved in protein-protein interactions. The CTD contains a conserved domain called the repressor domain, which regulates the pore-forming activity of the protein.

Activity of Recombinant Mouse GSDMD/Gasdermin-D Protein

GSDMD/Gasdermin-D protein is primarily known for its role in pyroptosis, a form of cell death that is triggered by inflammatory signals. In its inactive form, GSDMD/Gasdermin-D protein is bound to the plasma membrane of cells. Upon activation by inflammatory signals, the protein undergoes cleavage by inflammatory caspases, resulting in the release of the N-terminal fragment. This fragment then forms pores in the plasma membrane, leading to cell death. In addition to pyroptosis, GSDMD/Gasdermin-D protein has also been implicated in other forms of cell death, such as apoptosis and necroptosis.

Application of Recombinant Mouse GSDMD/Gasdermin-D Protein

The recombinant form of GSDMD/Gasdermin-D protein has a wide range of applications in the fields of immunology and cell biology. One of its main applications is in the study of pyroptosis and other forms of cell death. Researchers can use recombinant GSDMD/Gasdermin-D protein to induce pyroptosis in cells and study its mechanisms. This can provide valuable insights into the role of this protein in various cellular processes and diseases.

Furthermore, recombinant GSDMD/Gasdermin-D protein can also be used as an antigen in immunological studies. Its ability to form pores in the plasma membrane makes it an excellent target for the immune system, making it a valuable tool for vaccine development. In addition, the protein can also be used in diagnostic tests for various diseases, as its levels have been found to be altered in certain conditions.

Moreover, recombinant GSDMD/Gasdermin-D protein has been shown to have potential therapeutic applications. Its role in inflammation and cell death makes it a promising target for the treatment of inflammatory diseases. In addition, its ability to induce cell death in cancer cells makes it a potential candidate for cancer therapy.

Conclusion

In summary, recombinant GSDMD/Gasdermin-D protein is a highly versatile protein with important roles in cell death, inflammation, and immunity. Its structure and activity have been extensively studied, and its potential applications in various fields make it a valuable tool for researchers. As our understanding of this protein continues to grow, it is likely that it will play an even greater role in advancing