Recombinant Mouse IL4 Protein, N-His-SUMO

Description of Recombinant Mouse IL4 Protein, N-His-SUMO

Introduction

Recombinant Mouse IL4 Protein, also known as Interleukin 4, is a cytokine that plays a crucial role in the immune response. It is a small protein with a molecular weight of approximately 15 kDa and is produced by various cell types, including T cells, B cells, and mast cells. Recombinant Mouse IL4 Protein is widely used in research and clinical applications due to its diverse functions and potential therapeutic applications.

Structure of Recombinant Mouse IL4 Protein

Recombinant Mouse IL4 Protein is a member of the IL4/IL13 family of cytokines and shares structural similarities with other members of the family, including IL13, IL5, and IL3. It is composed of 129 amino acids and has a four-helix bundle structure, with two long helices connected by two shorter helices. The protein is highly conserved among different species, with a 60% sequence homology between mouse and human IL4.

The crystal structure of Recombinant Mouse IL4 Protein has been extensively studied, revealing key structural features that are essential for its biological activity. The protein has a hydrophobic core and a hydrophilic surface, with the latter containing the receptor-binding site. The receptor-binding site is composed of three loops, known as A, B, and C, which interact with the IL4 receptor (IL4R) and IL13 receptor (IL13R) to initiate signaling.

Activity of Recombinant Mouse IL4 Protein

Recombinant Mouse IL4 Protein is a pleiotropic cytokine that exerts its effects on a wide range of cell types. It is primarily known for its role in promoting Th2 immune responses, but it also has anti-inflammatory and immunosuppressive activities. The main function of IL4 is to stimulate the differentiation of naive CD4+ T cells into Th2 cells, which produce cytokines such as IL4, IL5, and IL13. These cytokines play a crucial role in the activation of B cells, leading to the production of antibodies, particularly IgE, which is involved in allergic reactions.

In addition to its effects on T cells and B cells, Recombinant Mouse IL4 Protein also has direct effects on other cell types, including macrophages, dendritic cells, and mast cells. It can promote the differentiation of monocytes into anti-inflammatory M2 macrophages, which play a crucial role in tissue repair and wound healing. IL4 also inhibits the production of pro-inflammatory cytokines by dendritic cells and promotes their differentiation into tolerogenic dendritic cells, which suppress immune responses. Furthermore, IL4 can activate mast cells, leading to the release of histamine and other mediators involved in allergic reactions.

Applications of Recombinant Mouse IL4 Protein

Recombinant Mouse IL4 Protein has numerous applications in both research and clinical settings. In research, it is commonly used to study the role of IL4 in various immune responses, including Th2 differentiation, B cell activation, and macrophage polarization. It is also used to investigate the mechanisms of allergic reactions and to develop new therapies for allergic diseases.

In the clinic, Recombinant Mouse IL4 Protein has potential therapeutic applications for various diseases, including asthma, allergies, and autoimmune disorders. IL4 has been shown to have anti-inflammatory and immunosuppressive effects, making it a promising candidate for the treatment of inflammatory diseases. Additionally, IL4 has been studied as a potential therapy for cancer, as it can inhibit the growth and survival of certain cancer cells.

Conclusion

Recombinant Mouse IL4 Protein is a small but powerful cytokine with diverse functions and potential therapeutic applications. Its structure and activity have been extensively studied, and it is widely used in research and clinical settings. As our understanding of the immune system and the role of IL4 continues to grow, Recombinant Mouse