4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
Brand
ProteoGenix
Host species
Escherichia coli (E.coli)
Fragment Type
Property sequence
Protein Accession
NP_734212.1
NCBI Reference
NP_734212.1
Aliases /Synonyms
GST-TEV, NIa-Pro protein (TEV protease)
Reference
PX-P1108
Note
For research use only
Description of Tobacco TEV Protease Recombinant Enzyme
General information on Tobacco TEV Protease Recombinant Enzyme:
TEV (Tobacco Etch Virus) Protease is a highly site-specific cysteine protease that is found in the Tobacco Etch Virus. Due to its high sequence specificity it is regularly used to cleave affinity tags from fusion proteins. The TEV encodes its whole genome as a single massive polyprotein. This is cleaved into functional units by the three proteases: P1 protease (1 cleavage site), helper-component protease (1 cleavage site) and TEV protease (7 cleavage sites). The greatest recognition site for this enzyme is the sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) [ENLYFQ/S] and cleavage appear between the Gln and Gly/Ser residues.
1: Martínez F, Daròs JA. Tobacco etch virus protein P1 traffics to the nucleolus
and associates with the host 60S ribosomal subunits during infection. J Virol.
2014 Sep;88(18):10725-37. doi: 10.1128/JVI.00928-14. Epub 2014 Jul 2. PubMed
PMID: 24991017; PubMed Central PMCID: PMC4178839.
2: Cui X, Wei T, Chowda-Reddy RV, Sun G, Wang A. The Tobacco etch virus P3
protein forms mobile inclusions via the early secretory pathway and traffics
along actin microfilaments. Virology. 2010 Feb 5;397(1):56-63. doi:
10.1016/j.virol.2009.11.015. Epub 2009 Nov 30. PubMed PMID: 19945728.
3: Torres-Barceló C, Martín S, Daròs JA, Elena SF. From hypo- to
hypersuppression: effect of amino acid substitutions on the RNA-silencing
suppressor activity of the Tobacco etch potyvirus HC-Pro. Genetics. 2008
Oct;180(2):1039-49. doi: 10.1534/genetics.108.091363. Epub 2008 Sep 9. PubMed
PMID: 18780745; PubMed Central PMCID: PMC2567354.
4: Allison RF, Dougherty WG, Parks TD, Willis L, Johnston RE, Kelly M, Armstrong
FB. Biochemical analysis of the capsid protein gene and capsid protein of tobacco
etch virus: N-terminal amino acids are located on the virion's surface. Virology.
1985 Dec;147(2):309-16. PubMed PMID: 18640560.
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