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Xenopus rtTA Recombinant Protein

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100ug, 50ug
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Product nameXenopus rtTA Recombinant Protein
Origin speciesXenopus
Expression systemProkaryotic expression
SequenceMGHHHHHHHHHHSSGHIDDDDKHNMSRLDKSKVINGALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHVKNKRALLDALPIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTRPTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTTDSMPPLLRQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPADALDDFDLDMLPADALDDFDLDMLPADALDDFDLDMLPG
Molecular weight30,56 kDa
Purity estimated90%
BufferPBS, imidazole 400mM
Formliquid
Delivery conditionDry Ice
Delivery lead time in business days3-5 days if in stock; 3-5 weeks if production needed
Storage condition4°C for short term (1 week), -20°C or -80°C for long term (avoid freezing/thawing cycles; addition of 20-40% glycerol improves cryoprotection)
BrandProteoGenix
Host speciesEscherichia coli (E.coli)
ApplicationsELISA,WB
Fragment TypeFull-length
Protein AccessionABC65842.1
NCBI ReferenceABC65842.1
Aliases /SynonymsrtTA, rtTA-M2.alt [Expression vector peTetOn(PacI)], doxycycline-activated transcriptional activator
ReferencePX-P1148
NoteFor research use only.

Description of Xenopus rtTA Recombinant Protein

General information on Xenopus rtTA Recombinant Protein

Fusion protein of reverse tetracycline repressor protein and immediate early protein 1 activation domain. Used in Tetracycline repressor-regulated gene repression experiments.

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Publication

1: Bäckman CM, Zhang Y, Hoffer BJ, Tomac AC. Tetracycline-inducible expression systems for the generation of transgenic animals: a comparison of various inducible systems carried in a single vector. J Neurosci Methods. 2004 Oct 30;139(2):257-62. PubMed PMID: 15488239.

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