Europe: 2-4 business days USA & Canada: 2-4 business days Rest of the world: 5-10 business days
Storage condition
4°C
Brand
ProteoGenix
Host species
Mammalian cells
Reference
PX-XTE-002
Note
For research use only.
Kit Content
1L bottle
Limited Use Label Licenses
Limited Use Label License for internal Research and Development Use of Mammalian Cell Lines
Description of XtenCHO ™ Expression medium
XtenCHO™ Expression Medium: Optimized for High-Yield CHO Cell Protein Production
The XtenCHO™ Expression Medium is specifically formulated for small to medium-scale recombinant protein production in Chinese Hamster Ovary (CHO) cells. This serum-free, animal-component-free, and chemically defined medium ensures high reproducibility and batch-to-batch consistency, providing researchers with a reliable and optimized culture environment. Its serum-free composition significantly enhances transfection efficiency, while its formulation supports balanced cell growth and viability in suspension cultures.
When used in combination with XtenCHO™ Race Cells and the XtenCHO™ Race Transfection Kit, this system has achieved an exceptional antibody production yield of 904 mg/L, making it a market leader in transient gene expression.
How to Use XtenCHO™ Expression Medium
The XtenCHO™ Expression Medium is designed for high-efficiency transient transfection, supporting volumes from a few milliliters to 10 liters. It serves both for transfection and cell sub-culturing, simplifying experimental workflows and reducing variability in conditions.
The XtenCHO™ Starter Kit includes comprehensive protocols for seamless integration into various lab setups, ensuring ease of use and adaptability.
Supplementation Requirements
For optimal transfection results, XtenCHO™ Expression Medium requires additional supplementation with:
L-Glutamine (8mM): A crucial nutrient for cell metabolism and growth. However, due to its rapid hydrolysis, regular supplementation is necessary to maintain its effective concentration and avoid toxic byproducts.
Anti-Clumping Agent (0.5%): Prevents cell aggregation in suspension cultures. However, it should not be added during transfection, as it can negatively impact protein yields.
Culture Conditions
For best results, use XtenCHO™ Expression Medium in:
-Sterile, vented, baffled Erlenmeyer shake flasks
-Incubator shaker set at 37°C, ≥80% relative humidity, and 5% CO₂
The XtenCHO™ Expression Medium is an essential component of the XtenCHO™ Race Starter Kit but is also available separately for worldwide delivery, ensuring flexibility for diverse research needs.
XtenCHO™ Race outperforms up to 6X (Cixutumumab and Peresolimab)
Crude production yields in mg/L obtained in 24-well plates cultures without feeding (Octet Analysis)
Yields in flask (better aeration) will be 1.5 to 2.5 Higher for Human/humanized IgG1/IgG4 (depending on the antibody considered)
Publication
Luo K, Chen Y, Wang F. Shrimp Plasma MANF Works as an Invertebrate Anti-Inflammatory Factor via a Conserved Receptor Tyrosine Phosphatase. J Immunol. 2022 Mar 1;208(5):1214-1223. doi: 10.4049/jimmunol.2100595. Epub 2022 Feb 11. PMID: 35149533.
Bonduelle O, Chaudesaigues C, Tolazzi M, Suleiman E, de Bernard S, Alves K, Nourikyan J, Bohec M, Baudrin LG, Katinger D, Debré P, Scarlatti G, Vieillard V, Combadière B. Dichotomy in Neutralizing Antibody Induction to Peptide-Conjugated Vaccine in Squalene Emulsion Contrast With Aluminum Hydroxide Formulation. Front Immunol. 2022 Apr 7;13:848571. doi: 10.3389/fimmu.2022.848571. PMID: 35464449; PMCID: PMC9021396.
Sandberg, A., Berenjeno-Correa, E., Rodriguez, R.C. et al. Aβ42 oligomer-specific antibody ALZ-201 reduces the neurotoxicity of Alzheimer’s disease brain extracts. Alz Res Therapy 14, 196 (2022). https://doi.org/10.1186/s13195-022-01141-1
Villatoro, A., Cuminetti, V., Bernal, A. et al. Endogenous IL-1 receptor antagonist restricts healthy and malignant myeloproliferation. Nat Commun 14, 12 (2023). https://doi.org/10.1038/s41467-022-35700-9
Michel Cogne, Natsuko UEDA, Marine CAHEN et al. Single-hit genome editing in B cells to redirect their specificity toward tumor antigens, 06 May 2024, PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-4317490/v1]
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