Human CD278/ICOS HEK293T Stable Cell Line

Title: Introduction to Human CD278/ICOS HEK293T Stable Cell Line

Human CD278, also known as inducible T-cell co-stimulator (ICOS), is a member of the CD28 superfamily of T-cell co-stimulatory molecules. It is a transmembrane glycoprotein expressed on activated T cells, and plays a critical role in regulating T-cell activation and differentiation. The Human CD278/ICOS HEK293T Stable Cell Line is a valuable tool for studying the structure, activity and application of this important immune checkpoint molecule.

Structure of Human CD278/ICOS

Human CD278/ICOS is a 55 kDa type I transmembrane protein that is composed of a single extracellular immunoglobulin (Ig) domain, a transmembrane domain, and a cytoplasmic tail. The extracellular domain contains a conserved cysteine-rich region and a binding site for the ligand B7-H2. The cytoplasmic tail contains two conserved signaling motifs, a YMFM motif that is important for binding to the signaling molecule PI3K, and a proline-rich motif that is essential for the recruitment of the adaptor protein SHP-2.

Activity of Human CD278/ICOS

Human CD278/ICOS is primarily expressed on activated T cells, but can also be found on other immune cells such as B cells, natural killer cells, and dendritic cells. It plays a crucial role in regulating T-cell activation and differentiation by providing a co-stimulatory signal to T cells. When the extracellular domain of CD278/ICOS binds to its ligand B7-H2 on antigen-presenting cells, it triggers a downstream signaling cascade that leads to the activation of PI3K and NF-κB pathways. This results in the production of cytokines, such as IL-4 and IL-10, which promote T-cell proliferation and differentiation.

Application of Human CD278/ICOS HEK293T Stable Cell Line

The Human CD278/ICOS HEK293T Stable Cell Line is a genetically modified cell line that stably expresses human CD278/ICOS. It is a valuable tool for studying the structure and function of this important immune checkpoint molecule. This cell line can be used in a variety of applications, including:

1. Flow cytometry analysis: The Human CD278/ICOS HEK293T Stable Cell Line can be used as a positive control in flow cytometry experiments to detect the expression of CD278/ICOS on T cells. This allows for the identification and characterization of CD278/ICOS-expressing cells in different tissues and under different experimental conditions.

2. Screening for therapeutic agents: CD278/ICOS has emerged as an attractive therapeutic target for the treatment of various autoimmune diseases and cancer. The Human CD278/ICOS HEK293T Stable Cell Line can be used in high-throughput screening assays to identify potential therapeutic agents that can modulate the activity of CD278/ICOS.

3. Studying the role of CD278/ICOS in T-cell activation and differentiation: The Human CD278/ICOS HEK293T Stable Cell Line can be used to investigate the signaling pathways and molecular mechanisms involved in CD278/ICOS-mediated T-cell activation and differentiation. This can provide valuable insights into the role of CD278/ICOS in immune responses and disease pathogenesis.

4. Development of novel immunotherapies: The Human CD278/ICOS HEK293T Stable Cell Line can be used to generate chimeric antigen receptor (CAR) T cells that express CD278/ICOS. These CAR T cells have shown promising results in preclinical studies as a potential treatment for cancer, and the Human CD278/ICOS HEK293T Stable Cell Line can be used to further optimize and develop this therapy.

In conclusion, the Human CD278/ICOS HEK293T Stable Cell Line is a valuable tool for studying the structure, activity and application of CD278/ICOS.