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12 culture conditions tested
Protein production in 4 weeks
Cleaner and safer protein with endotoxin-free system
Bacillus subtilis protein expression service with 12 culture conditions screened to maximize your protein expression success
12 culture conditions are screened in parallel to identify the most suitable production strategy, even for complex targets. Increase your chances of expressing your protein under the right conditions from the start.
Get your purified protein faster in 4 weeks
Receive your custom protein samples, purified and delivered in 4 weeks. Save valuable time with one integrated workflow, from codon optimization and gene design to protein production.
Expression vector construction:
- Gene design including codon optimization
- Gene subcloning into a high-yield expression vector
Preliminary study: screening of 12 conditions and optimization to assess production parameters and determine the best protein expression conditions.
Small-scale protein expression and purification:
- Protein expression under the previously optimized conditions
- Purification testing
Quality control and analysis:
- SDS-PAGE and Western Blot analysis
- Quantification using the Bradford mathod
Data report
Get free access to representative data
from a B. subtilis expression & purification test project
You receive a comprehensive report with detailed data, advanced test results, and key insights throughout the process.
Bacillus subtilis protein expression service for cleaner and safer proteins
Use an endotoxin-free alternative to E. coli, with the potential to secrete your target protein directly into the culture medium. You reduce purification constraints and gain better sample quality.
Move to larger protein volumes with one partner
Scale up your protein production from microgram to gram quantities in 1 week. Benefit from a reliable transition across production volumes with a single partner.
Our approach can support custom cell line development to enable industrial-scale production, which requires a switch to a mammalian expression system.
A backup plan if it doesn’t work in B. subtilis
As an alternative strategy for your protein, if B. subtilis does not deliver the expected results, we can propose the following expression systems:
FAQ
