Introduction
Human CD112, also known as Nectin-2 or PVRL2, is a cell adhesion molecule that belongs to the immunoglobulin superfamily. It is expressed on the surface of various cell types, including immune cells, and plays a crucial role in cell-cell interactions. The HEK293T stable cell line expressing human CD112 is a valuable tool for studying the structure, activity and potential therapeutic applications of this important molecule.
Structure of Human CD112
Human CD112 is a type I transmembrane protein with a molecular weight of approximately 65 kDa. It consists of a single extracellular domain, a transmembrane region, and a cytoplasmic tail. The extracellular domain of CD112 contains three immunoglobulin-like domains (V, C1, and C2) that are involved in its binding to other molecules. The cytoplasmic tail of CD112 contains a conserved tyrosine residue that can be phosphorylated and is important for signal transduction.
Activity of Human CD112
CD112 is primarily known for its role in cell adhesion and immune cell activation. It interacts with several other molecules, including CD226, CD96, and TIGIT, to mediate cell-cell interactions. These interactions are important for the activation and regulation of immune responses, including T cell and natural killer (NK) cell activity.
In addition to its adhesive functions, CD112 also has signaling capabilities. Upon binding to its receptors, CD112 can activate intracellular signaling pathways, such as the PI3K-Akt and MAPK pathways, leading to cell proliferation and survival. This signaling activity of CD112 is important for its role in immune cell activation and regulation.
Application of Human CD112 HEK293T Stable Cell Line
The HEK293T stable cell line expressing human CD112 is a valuable tool for studying the function and potential therapeutic applications of this molecule. The stable expression of CD112 in this cell line ensures consistent and reliable results, making it ideal for research and drug discovery.
One application of this cell line is in flow cytometry. The stable expression of CD112 on the surface of HEK293T cells allows for the detection and quantification of CD112 expression levels. This can be useful in studying the regulation of CD112 expression and its role in immune cell activation.
Moreover, the HEK293T stable cell line expressing human CD112 can be used as a model system for studying the interactions between CD112 and its receptors, such as CD226 and TIGIT. This can provide insights into the signaling mechanisms and functional consequences of these interactions.
Finally, this cell line can also be used for screening and testing potential therapeutic agents targeting CD112. By studying the effects of these agents on CD112-expressing cells, researchers can identify potential drugs for the treatment of immune-related disorders, such as autoimmune diseases and cancer.
Conclusion
In summary, the HEK293T stable cell line expressing human CD112 is a valuable tool for studying the structure, activity and potential therapeutic applications of this important cell adhesion molecule. Its stable expression of CD112 allows for reliable and consistent results, making it ideal for various research and drug discovery applications. With its role in immune cell activation and regulation, CD112 remains a promising therapeutic target for the treatment of various diseases.
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