Human CD326/EPCAM HEK293T Stable Cell Line

Introduction

The Human CD326/EPCAM HEK293T Stable Cell Line is a valuable tool for researchers in the field of cancer biology and immunotherapy. This cell line is derived from HEK293T cells, which are human embryonic kidney cells that have been transformed with the SV40 large T antigen. The stable expression of CD326/EPCAM in these cells allows for the study of this protein’s structure, activity, and potential therapeutic applications.

Structure of CD326/EPCAM

CD326/EPCAM, also known as Epithelial Cell Adhesion Molecule (EpCAM), is a transmembrane glycoprotein that is highly expressed in epithelial cells and some tumors. It consists of an extracellular region, a transmembrane domain, and a cytoplasmic tail. The extracellular region contains two epidermal growth factor (EGF)-like domains and a thyroglobulin type-1 (TY) domain, which are involved in cell adhesion and signaling. The cytoplasmic tail contains a PDZ-binding motif, which interacts with intracellular signaling molecules.

Activity of CD326/EPCAM

CD326/EPCAM plays a crucial role in cell adhesion, proliferation, and differentiation. It is involved in maintaining the integrity of epithelial tissues by mediating cell-cell adhesion through homophilic interactions. It also regulates cell signaling pathways, such as the Wnt/β-catenin pathway, which is important for cell proliferation and differentiation. Additionally, CD326/EPCAM has been shown to play a role in cancer progression and metastasis by promoting tumor cell migration and invasion.

Application in Flow Cytometry

The Human CD326/EPCAM HEK293T Stable Cell Line is a valuable tool for flow cytometry studies. Flow cytometry is a technique used to analyze and sort cells based on their physical and chemical properties. The stable expression of CD326/EPCAM in HEK293T cells allows for the specific labeling and isolation of cells expressing this protein. This is particularly useful for studying the distribution and function of CD326/EPCAM in different cell types and tissues.

Therapeutic Target

CD326/EPCAM has been identified as a potential therapeutic target in cancer treatment. It is highly expressed in many types of cancer, including breast, colon, and lung cancer, and has been associated with poor prognosis. Targeting CD326/EPCAM has been shown to inhibit tumor growth and metastasis in preclinical studies. The Human CD326/EPCAM HEK293T Stable Cell Line can be used to screen for potential therapeutic agents that target this protein and to study their mechanisms of action.

Conclusion

In summary, the Human CD326/EPCAM HEK293T Stable Cell Line is a valuable tool for researchers studying CD326/EPCAM in cancer biology and immunotherapy. Its stable expression of CD326/EPCAM allows for the study of the protein’s structure, activity, and potential therapeutic applications. Additionally, this cell line is a useful tool for flow cytometry studies and can be used as a model for screening potential therapeutic agents targeting CD326/EPCAM. Further research using this cell line may lead to the development of novel treatments for cancer.