Which expression systems do you use for transient expression (e.g., HEK293, CHO, other proprietary lines)?

We primarily use XtenCHO™, our CHO-based transient expression platform designed for robust yields and consistent protein quality. If your project requires a specific host or expression context, we can advise on the best-fit system during project scoping.

Can you handle difficult-to-express or aggregation-prone proteins?

Yes. We have strong expertise in challenging targets, including aggregation-prone proteins and other difficult-to-express formats. We can recommend risk-mitigation strategies (construct design, tags, expression conditions, purification approach) to maximize solubility and recovery.

What is the typical turnaround time from DNA to purified protein?

Typical turnaround is ~15 days from DNA/plasmid receipt and project kickoff to purified protein, depending on the protein format and requested analyses.

Do you offer DNA-to-protein workflows, or do I need to provide plasmids?

Both options are available: DNA-to-protein: you provide the sequence, we handle cloning/expression/purification. Plasmid-to-protein: you provide plasmids, we proceed directly to expression and purification.

How scalable is the transient expression process—from milligram to gram quantities?

Our process supports a wide production range, from microgram quantities up to gram-scale output, depending on the target and project requirements.

What quality control and analytical methods are included?

Standard QC includes: Protein quantification (Bradford assay or UV280) SDS-PAGE (qualitative assessment) Purity evaluation Additional analytics can be discussed depending on your downstream needs.

Do you provide recommendations for construct optimization or expression conditions?

Yes. We provide construct and condition recommendations to improve expression, stability, and purity (e.g., sequence/construct choices, tags, secretion strategy, expression parameters).

Do you offer additional services such as stable cell line development, protein engineering, or antibody production?

Yes. We offer stable cell line development, protein engineering, and antibody production, along with a broad portfolio of other bioreagent and custom R&D services (as listed on the ProteoGenix website).

Are your labs compliant with relevant quality or regulatory standards (ISO, GMP-like)?

Yes. Our work is supported by an ISO-based quality system and GMP-like practices appropriate for research-grade production and documentation needs.

Can you offer continuity for follow-up studies, variant screening, or scale-up?

Absolutely. Each client is assigned a dedicated PhD-level scientific support who follows the project end-to-end, enabling smooth continuity for follow-up studies, variant screening, re-runs, and scale-up.

Small-scale transient protein expression service

Produce from µg to g as little as 15 business days

Starting at a 15-day turnaround time

Adapted for complex targets

Proteins with >95% Purity

No Packages. Just pay for what your protein really needs

Every protein has unique requirements to fit your specific application. With no preset packages, together we design your protein production service so you only pay for what your protein truly needs, while fitting your budget and schedule.

Five expression systems to find the perfect fit for your protein

With five expression systems, you ensure the perfect balance to maximize yield and activity while staying on a budget. A PhD project manager will guide you, so you’re never on your own.

Produce complex eukaryotic proteins with native folding and PTMs. Perfect for in vivo use with low endotoxins.

Mammalian protein expression service
Get fast, high-yield expression for simple proteins, ideal for structural studies.

E-coli protein expression services

Multi-System Expression Evaluation

Screen up to five systems to quickly find the one with the best yield and activity for your protein.

Your purified protein in 15 business days

Transient protein expression process

Gene synthesis

- Subcloning into an expression vector

Recombinant production and purification

- Plasmid amplification and preparation

- Transfection

- Transient expression

- Purification

Expression evaluation

- SDS-PAGE analysis

One-step purification

Quality control

- Quantification by Bradford or UV280

- Qualitative SDS-PAGE analysis

- Purity evaluation

Yes. We have strong expertise in challenging targets, including aggregation-prone proteins and other difficult-to-express formats. We can recommend risk-mitigation strategies (construct design, tags, expression conditions, purification approach) to maximize solubility and recovery.
Both options are available:
- DNA-to-protein: you provide the sequence, we handle cloning/expression/purification.
- Plasmid-to-protein: you provide plasmids, we proceed directly to expression and purification.
Standard QC includes:
- Protein quantification (Bradford assay or UV280)
- SDS-PAGE (qualitative assessment)
- Purity evaluation
Additional analytics can be discussed depending on your downstream needs.

Related Services

Custom recombinant antibody production High throughput antibody production service Transient expression services

Small-scale transient protein expression service