Recombinant Human CCT3 Protein, N-His

Description of Recombinant Human CCT3 Protein, N-His

Introduction

Recombinant Human CCT3 Protein is a highly versatile and essential protein that plays a crucial role in various cellular processes. This protein is a member of the chaperonin-containing TCP-1 (CCT) complex, which is responsible for the folding and assembly of many proteins in the cell. In this article, we will explore the structure, activity, and applications of this important recombinant protein.

Structure of Recombinant Human CCT3 Protein

Recombinant Human CCT3 Protein is a 60-kDa protein that consists of 547 amino acids. It is composed of three distinct domains – the N-terminal domain, the intermediate domain, and the C-terminal domain. The N-terminal domain is responsible for binding to the other subunits of the CCT complex, while the intermediate domain is involved in the ATPase activity of the protein. The C-terminal domain is responsible for substrate binding and protein folding.

The CCT complex is composed of eight subunits, with CCT3 being one of them. These subunits form a double-ring structure, with each ring consisting of four subunits. The two rings are stacked on top of each other, creating a central cavity where protein folding takes place. The CCT complex also has a lid-like structure that covers the central cavity, providing a protected environment for protein folding.

Activity of Recombinant Human CCT3 Protein

The main function of Recombinant Human CCT3 Protein is to assist in the folding and assembly of other proteins in the cell. It acts as a molecular chaperone, which means it helps other proteins to fold correctly and prevent them from forming non-functional or harmful structures. This is essential for the proper functioning of the cell, as misfolded proteins can lead to various diseases.

The activity of Recombinant Human CCT3 Protein is dependent on the hydrolysis of ATP. The protein uses the energy from ATP hydrolysis to undergo conformational changes, which are necessary for the folding of other proteins. The CCT complex also has the ability to recognize and bind to specific sequences of amino acids in the target protein, aiding in its proper folding.

Applications of Recombinant Human CCT3 Protein

Recombinant Human CCT3 Protein has various applications in both research and industrial settings. One of its primary uses is in the production of recombinant proteins. The CCT complex is often co-expressed with the target protein in recombinant protein production systems, where it helps in the proper folding and assembly of the recombinant protein. This results in higher yields of correctly folded and functional recombinant proteins.

Additionally, Recombinant Human CCT3 Protein has been studied for its potential role in protein misfolding diseases, such as Alzheimer’s and Parkinson’s. It has been shown to interact with proteins involved in these diseases and may play a role in their proper folding. This makes it a potential target for therapeutic interventions in the future.

Moreover, the CCT complex has been found to be essential for the growth and survival of cancer cells. Inhibition of CCT activity has been shown to lead to cell death in cancer cells, making it a potential target for cancer treatment.

Conclusion

In conclusion, Recombinant Human CCT3 Protein is a crucial protein in the cell that is involved in protein folding and assembly. Its structure and activity make it an essential component of the CCT complex, which is responsible for proper protein folding. The applications of this protein range from recombinant protein production to potential therapeutic interventions in diseases such as cancer. Further research on this protein and its complex may provide valuable insights into its role in cellular processes and potential therapeutic applications.