Human CD324/CDH1 HEK293T Stable Cell Line

Introduction

The Human CD324/CDH1 HEK293T Stable Cell Line is a valuable tool for studying the structure, activity and applications of the CD324/CDH1 protein in a controlled laboratory setting. This stable cell line is derived from HEK293T cells, a popular cell line commonly used in biomedical research. The CD324/CDH1 protein, also known as E-cadherin, is a cell adhesion molecule that plays a critical role in maintaining the integrity and function of epithelial tissues. In this article, we will discuss the structure, activity and potential applications of the Human CD324/CDH1 HEK293T Stable Cell Line.

Structure of CD324/CDH1 protein

The CD324/CDH1 protein is a transmembrane glycoprotein composed of 882 amino acids. It is a member of the cadherin superfamily, which are calcium-dependent cell adhesion molecules. The extracellular region of CD324/CDH1 contains five cadherin repeats, which are responsible for the homophilic binding of CD324/CDH1 to other CD324/CDH1 molecules on neighboring cells. The intracellular region of CD324/CDH1 interacts with various cytoplasmic proteins, such as β-catenin, to mediate signaling pathways involved in cell adhesion, cell migration, and tissue organization.

Activity of CD324/CDH1 protein

The activity of CD324/CDH1 is regulated by various factors, including calcium concentration, phosphorylation, and protein-protein interactions. Calcium ions play a critical role in stabilizing the homophilic binding of CD324/CDH1, thus promoting cell-cell adhesion. Phosphorylation of CD324/CDH1 can modulate its activity by altering its binding affinity to other proteins. For example, phosphorylation of CD324/CDH1 by protein kinase C (PKC) can disrupt its interaction with β-catenin, leading to the disassembly of adherens junctions and increased cell motility. In addition, CD324/CDH1 can interact with other signaling molecules, such as growth factor receptors, to regulate cell proliferation and survival.

Applications of Human CD324/CDH1 HEK293T Stable Cell Line

The Human CD324/CDH1 HEK293T Stable Cell Line has various applications in biomedical research, particularly in the fields of cancer biology and drug discovery. Here are some potential applications of this stable cell line:

1. Study of epithelial-mesenchymal transition (EMT)

EMT is a cellular process in which epithelial cells lose their cell-cell adhesion and acquire a mesenchymal phenotype, allowing them to migrate and invade surrounding tissues. EMT is a critical step in cancer metastasis and is associated with the loss of CD324/CDH1 expression. The Human CD324/CDH1 HEK293T Stable Cell Line can be used to study the molecular mechanisms underlying EMT and to screen for potential inhibitors of this process.

2. Identification of therapeutic targets

CD324/CDH1 has been identified as a potential therapeutic target in various types of cancer, including breast, lung, and colon cancer. The Human CD324/CDH1 HEK293T Stable Cell Line can be used to validate the role of CD324/CDH1 in cancer progression and to identify potential therapeutic agents that can target this protein.

3. Drug screening and toxicity testing

The Human CD324/CDH1 HEK293T Stable Cell Line can be used in drug screening assays to identify compounds that can modulate the activity of CD324/CDH1 or its downstream signaling pathways.