Recombinant Human ABCB6, N-His

Description of Recombinant Human ABCB6, N-His

Introduction to Recombinant Human ABCB6

Recombinant Human ABCB6 is a protein that plays a crucial role in the transport of molecules across cell membranes. This protein is a member of the ATP-binding cassette (ABC) transporter family, which is responsible for the movement of various substances in and out of cells. The ABCB6 protein is encoded by the ABCB6 gene, and its recombinant form is produced through genetic engineering techniques. In this article, we will explore the structure, activity, and applications of Recombinant Human ABCB6.

Structure of Recombinant Human ABCB6

The ABCB6 protein is composed of 678 amino acids and has a molecular weight of approximately 75 kDa. It contains two transmembrane domains and two nucleotide-binding domains, which are connected by a linker region. The transmembrane domains are responsible for the transport of molecules across the cell membrane, while the nucleotide-binding domains bind to ATP, providing the energy for the transport process.

The recombinant form of ABCB6 is produced by inserting the DNA sequence of the ABCB6 gene into a suitable expression vector, which is then introduced into host cells for protein production. The resulting protein is identical to the native form and retains its structure and function.

Activity of Recombinant Human ABCB6

The main function of ABCB6 is to transport molecules across cell membranes, including drugs, lipids, and other small molecules. It is also involved in heme biosynthesis, the process of producing heme, a component of hemoglobin responsible for binding and transporting oxygen in the blood.

The activity of ABCB6 is regulated by various factors, including the concentration of ATP, pH, and the presence of other transport proteins. The binding of ATP to the nucleotide-binding domains induces a conformational change in the protein, allowing it to bind to its substrate and transport it across the membrane.

Studies have also shown that ABCB6 plays a role in drug resistance in cancer cells. It is overexpressed in certain types of cancer, and its increased activity can lead to the efflux of chemotherapeutic drugs, reducing their effectiveness. This highlights the importance of understanding the structure and activity of this protein for the development of new cancer treatments.

Applications of Recombinant Human ABCB6

Recombinant Human ABCB6 has a wide range of potential applications, particularly in the field of drug discovery and development. Its role in drug transport and resistance makes it a promising target for the development of new drugs that can modulate its activity.

Additionally, the production of recombinant ABCB6 in large quantities allows for its use in biochemical and biophysical studies to further understand its structure and function. This can aid in the development of more effective drugs that can target this protein and potentially overcome drug resistance in cancer cells.

Furthermore, recombinant ABCB6 can also be used as an antigen in the production of antibodies for research and diagnostic purposes. Antibodies against this protein can be used to detect its expression in different tissues and cells, providing insight into its role in various physiological processes.

Conclusion

In conclusion, Recombinant Human ABCB6 is a vital protein involved in the transport of molecules across cell membranes. Its structure, activity, and applications make it a promising target for drug development and a valuable tool for research and diagnostics. With further studies and advancements in technology, the potential of this protein in various fields is yet to be fully explored.