Recombinant Human ASIC2 Protein, N-His

Description of Recombinant Human ASIC2 Protein, N-His

Introduction

Recombinant Human ASIC2 Protein is a protein that is produced through recombinant DNA technology. It is a synthetic version of the human ASIC2 protein, which is a member of the acid-sensing ion channel (ASIC) family. This protein plays a crucial role in various physiological processes and has been extensively studied for its structure, activity, and potential applications.

Structure of Recombinant Human ASIC2 Protein

The recombinant human ASIC2 protein is composed of 524 amino acids and has a molecular weight of 59.7 kDa. It has a similar structure to the native human ASIC2 protein, with two transmembrane domains, a large extracellular loop, and intracellular N and C termini. The extracellular loop contains several cysteine residues that are important for protein stability and function.

Activity of Recombinant Human ASIC2 Protein

The recombinant human ASIC2 protein is a ligand-gated ion channel that is activated by extracellular protons. It is primarily expressed in the central nervous system and has been shown to play a role in pain sensation, learning and memory, and synaptic plasticity. Studies have also suggested its involvement in various diseases, such as epilepsy, anxiety, and depression.

The activity of the recombinant human ASIC2 protein can be modulated by various factors, including pH, temperature, and pharmacological agents. It has been shown to be sensitive to amiloride, a diuretic drug, and psalmotoxin, a peptide toxin from spider venom. These modulators can either enhance or inhibit the activity of the protein, making it a potential target for drug development.

Applications of Recombinant Human ASIC2 Protein

Due to its involvement in various physiological and pathological processes, the recombinant human ASIC2 protein has potential applications in both research and medicine. One of its main applications is in the study of pain sensation and the development of pain-relieving drugs. The protein has been shown to play a role in inflammatory and neuropathic pain, making it a promising target for the treatment of chronic pain conditions.

Moreover, the recombinant human ASIC2 protein has also been studied for its role in learning and memory. It has been shown to be involved in fear conditioning and spatial memory, making it a potential target for cognitive enhancers. Additionally, the protein has been linked to various neurological disorders, such as epilepsy and anxiety. By understanding its structure and activity, researchers can develop targeted therapies for these conditions.

Conclusion

In summary, Recombinant Human ASIC2 Protein is a synthetic version of the human ASIC2 protein that has been produced through recombinant DNA technology. Its structure and activity have been extensively studied, and it has shown potential applications in pain relief, cognitive enhancement, and treatment of neurological disorders. Further research on this protein may lead to the development of novel therapeutics for various diseases.